香菇尿嘧啶营养缺陷型菌株的筛选与分子验证  

Selected and molecular verification for uracil auxotrophic mutants of Lentinula edodes

作　　者：王连会[1,2] 茅文俊[1] 鲍大鹏[1] 

机构地区：[1]上海市农业科学院食用菌研究所,农业部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,上海市农业遗传育种重点开放实验室,上海201403 [2]上海海洋大学食品学院,上海201306

出　　处：《上海农业学报》2014年第3期6-9,共4页Acta Agriculturae Shanghai

基　　金：上海市科委基础研究重点项目"香菇安全稳定的同源转化体系的建立"(08JC1418100)资助

摘　　要：筛选出对5'-FOA敏感性较高的香菇‘L26'菌株,采用原生质体紫外诱变技术,获得‘L26'菌株18株尿嘧啶营养缺陷型突变菌株。分子验证表明:其中8株菌株的尿嘧啶合成代谢路径发生了基因突变。6个菌株的pyrF基因发生突变,2个菌株的pyrG基因发生突变。The ‘L26’strain of L .edodes with higher sensitivity of 5 ’-FOA was selected,and 18 uracil auxotrophic mutant strains were obtained by UV mutagenesis of protoplast.Molecular verification showed that 8 mutant strains had genetic mutations in uracil synthetic metabolic pathways;there were pyrF gene mutations in 6 strains and pyrG gene mutations in 2 strains.

关 键 词：香菇 尿嘧啶营养缺陷型 5’-氟乳清酸 

分 类 号：S646[农业科学—蔬菜学] Q78[农业科学—园艺学]