抑制 PKD2介导8-硝基白杨素诱导 U937细胞凋亡  

Inhibition of PKD2 mediated apoptosis induced by 5, 7-dihydrox-8-nitrochrysin in U937 cell line

作　　者：杨剑岭 曹晓正 何丽华[2] 曹建国[2] 杨剑锋[2] 

机构地区：[1]湖南省荣军医院儿科,长沙410119 [2]湖南师范大学医学院药物工程实验室

出　　处：《中国医师杂志》2014年第6期728-730,共3页Journal of Chinese Physician

基　　金：湖南省自然基金项目（03JJY5009）

摘　　要：目的：研究8-硝基白杨素（ NOC）诱导单核细胞白血病U937细胞凋亡作用是否涉及调控蛋白激酶D2（ PKD2）活性。方法体外培养U937细胞系细胞。碘化丙啶（PI）染色流式细胞术（FCM）分析细胞凋亡率。 DNA琼脂糖凝胶电泳检定细胞DNA梯形条带图谱。 Western blot分析PDK2磷酸化蛋白表达。结果 NOC（2.5、5.0、10.0μmol/L）增高U937细胞凋亡率，呈浓度依赖性（ P ＜0.05）。 NOC（5.0和10.0μmol/L）处理24 h，U937细胞呈现典型DNA梯形条带图谱。同时，NOC有效下调磷酸化PDK2蛋白表达；并与PKD2特异性抑制剂Goe6976协同增高U937细胞凋亡率。结论 NOC诱导U937细胞凋亡作用与其抑制PKD2激酶活性相关。Objective To investigate whether 5, 7-dihydrox-8-nitrochrysin （NOC） induces apoptosis in U937 monocytic leukae-mia cells is involved in the regulation of the activity of PKD 2.Methods U937 cell line cells were cultured in vitro .Apoptosis rate was analyzed by flow cytometry （FCM） using propidium iodide （PI） staining.DNA ladder bands were observed by DNA agarose gel electro-phoresis.The phosphorylated protein expression of PKD 2 was analyzed using Western blot .Results NOC （2.5, 5.0, and 10.0μmol/L） increased apoptosis rate in U937 cells in a concentration-dependent manner （ P 〈0.05）.After treatment with NOC （5.0 and 10.0μmol/L） for 24 h, U937 cells presented typical DNA ladder bands .At the same time, not only did NOC effectively down-regulate the ex-pression of PDK2 phosphorylated protein , but also increased apoptosis rate in U 937 cells in the presence of G？6976, a specific inhibitor of PKD2.Conclusions The effect that NOC induces apoptosis in U 937 cells is related to the inhibition of the activity of PKD 2.

关 键 词：蛋白激酶类 植物提取物 白血病 单核细胞 急性 细胞凋亡 

分 类 号：R733.7[医药卫生—肿瘤]