牙龈卟啉单胞菌临床菌株毒力相关性质分析  

Analysis of virulence-related properties of P.gingivalis clinical isolates

作　　者：周彦玢[1] 刘大力[1] 李云鹏[1] 王依玮[1] 束蓉[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院·口腔医学院牙周病科.上海市口腔医学重点实验室,上海200011

出　　处：《上海口腔医学》2014年第3期266-272,共7页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(30901672);高等学校博士学科点专项科研基金(20090073120096);上海交通大学医学院博士创新基金(BXJ201230)~~

摘　　要：目的:以模式菌株作为对照,对来自中国人口腔牙龈卟啉单胞菌(Porphyromonas gingivalis,P.gingivalis)临床菌株进行多种致病性质的分析,对致病性质与其毒力高低之间的关系进行初步探讨。方法:在前期对临床菌株毒力性质分析的基础上,检测和比较各菌株黏附宿主细胞、牙龈素活性以及抵抗宿主免疫吞噬等致病性质。采用SAS 8.02软件包对数据进行统计学分析。结果:P.gingivalis低毒模式菌株表现出较高的黏附红细胞及较弱的抵抗宿主血清杀伤的能力,高毒模式菌株表现出较弱的黏附红细胞及较强的抵抗血清杀伤的能力;但临床菌株的表现却与模式菌株相反。另外,P.gingivalis菌株牙龈素活性与毒力性质间无明显关系。结论:P.gingivalis毒力性质的多样性与其致病性质之间的关系不明确。PURPOSE： To analyze the relation of virulence properties and pathogenicity of Porphyromonos gingivalis （P. gingivals） isolated from Chinese patients. METHODS： Based on the previous analysis of virulence properties, investigations of pathogenicity properties, including the adhesion to host cells, gingipain activities, and resistance to host immune reaction were performed to analyze the diversity of pathogenic properties of these strains. SAS 8.02 software package was used for statistical analyses. RESULTS： Less-virulent type strain showed higher adherence ability to host red blood cells and weaker resistance to serum killing, while virulent type strain showed the opposite reactions. However, the clinic isolates presented just the opposite relationship between virulence properties and pathogenicity. In addition, there was no obvious correlation between gingipain activities and virulence properties of P. gingivalis strains. CONCLUSIONS： The results suggested that there is no obvious linkage between the P. gingivaliz virulence properties and pathogenicity diversity.

关 键 词：牙龈卟啉单胞菌 临床菌株 致病性质 

分 类 号：R781.4[医药卫生—口腔医学]