柚皮苷对犬骨髓基质细胞体外增殖及成骨分化的影响  

Effect of Naringin on proliferation and osteogenic differentiation of bone marrow stromal cells in vitro

作　　者：张学斌[1,2] 刘天麟[2] 张忻[1] 谭鸾君[2] 葛海良[3] 黄远亮[2] 

机构地区：[1]同济大学口腔医学院口腔生物医学及转化医学实验室,上海200072 [2]同济大学附属东方医院口腔科,上海200120 [3]上海交通大学医学院免疫学教研室,上海200025

出　　处：《上海口腔医学》2014年第3期280-284,共5页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(81070806);上海市科学技术委员会资助项目(10JC1413300);浦东新区卫生局青年项目(PW2011B-13)~~

摘　　要：目的:研究犬骨髓基质细胞(bone marrow stromal cells,BMSCs)在柚皮苷诱导下定向成骨及成骨分化能力。方法:抽取犬骨髓,贴壁法体外培养,流式细胞仪鉴定,加入不同浓度柚皮苷(1×10-5、1×10-6、1×10-7、1×10-8、1×10-9 mol/L)诱导,以CCK-8检测药物毒性,定量检测碱性磷酸酶、von Kossa检测钙结节形成。采用SPSS20.0软件包对数据进行统计学分析。结果:经流式细胞仪检测,CD34、CD45低表达、CD90高表达分别为0.126%、0.075%和95.4%;柚皮苷浓度在10-6、10-7 mol/L时,能明显促进细胞增殖;经柚皮苷诱导后,碱性磷酸酶含量显著提高,其中,浓度为10-6 mol/L时,ALP相对值最高(P<0.05);von Kossa钙结节检测呈阳性。结论:犬骨髓基质细胞在柚皮苷诱导下能分化为成骨细胞。柚皮苷浓度为10-6 mol/L时,能明显促进骨髓基质细胞的增殖及分化。PURPOSE： To investigate the differentiation and osteogenic activity of Naringin-induced bone marrow stromal cells （BMSCs） in dogs. METHODS：BMSCs were separated and cultured in vitro and identified by FCM. Then different concentration of Naringin （1×10^-5、1×10^-6、1×10^-7、1×10^-8、1×10^-9 mol/L） were added to cell culture media to induce BMSCs. The effect of Naringin on BMSCs was evaluated respectively by CCK-8 method and measuring the activity of alkaline phosphatase （ALP）. The formation of nodules of calcium was detected by yon Kossa staining. The data was analyzed with SPSS20.0 software package. RESULTS： The result of cell-surface marker displayed that the expression of CD34 and CD45 were negative while the expression of CD90 was positive. The values were 0.126%, 0.075% and 95.4%, respectively. Naringin could obviously promote cell proliferation, which exhibited the best effect on proliferation and osteogenic differentiation at concentration of 10 ^-6 mol/L. Calcium nodule （yon Kossa） staining was positive. CONCLUSIONS： Naringin-indueed bone marrow stromal cells can be differentiated into osteoblasts. Naringin at the concentration of 10^-6mol/L can enhance the proliferation and osteogenic differentiation of BMSCs.

关 键 词：柚皮苷 骨髓基质细胞 成骨诱导 骨组织工程 

分 类 号：R782[医药卫生—口腔医学]