羟喜树碱对人肾上腺皮质癌裸鼠移植瘤的抗瘤活性研究  

Antitumor Effects of 10-Hydroxycamptothecin Against Human Adrenocortical Carcinoma SW-13 Xenografts in Nude Mice

作　　者：文静[1] 张劼[1] 罗佐杰[1] 梁杏欢[1] 尹健兰 

机构地区：[1]广西医科大学第一附属医院内分泌科,南宁市530021

出　　处：《广西医学》2014年第6期701-703,共3页Guangxi Medical Journal

基　　金：国家自然科学基金资助项目(81060220);广西自然科学基金资助项目(2010GXNSFB013062);广西医药卫生科研课题(Z2013037)

摘　　要：目的观察羟喜树碱对人肾上腺皮质癌SW-13细胞裸鼠移植瘤的作用。方法裸鼠腋窝皮下接种人肾上腺皮质癌SW-13细胞,造模成功后随机分为对照组、干预A组和干预B组,每组8只,分别给予腹腔注射生理盐水、0.30 mg/ml羟喜树碱溶液和0.45 mg/ml羟喜树碱溶液,注射体积均为10 ml/kg,1次/d,每周连用5 d停2 d,总疗程2周。测量并绘制移植瘤生长曲线和无事件生存曲线,计算各干预组最佳相对肿瘤增长率(%T/C)、无事件生存期(EFS)T/C值。结果各干预组移植瘤生长曲线均较对照组平缓且出现生长延迟。对照组中位无事件生存期为5 d,干预A组为8 d,B组为17 d。两个干预组的最佳%T/C均<40%,羟喜树碱干预有效。干预A组的EFS T/C值为1.75,该浓度羟喜树碱具有低度抗瘤活性;干预B组的EFS T/C值为3.25,而移植瘤体积无净减小,具有中度抗瘤活性。结论羟喜树碱对人肾上腺皮质癌SW-13细胞裸鼠移植瘤具有一定抗瘤活性,可抑制瘤体生长,其浓度对抗瘤活性的影响有待进一步研究。Objective To investigate the antitumor effects of 10-hydroxycamptothecin （ HCPT ） against human adrenocortical carcinoma SW-13 xenografts in nude mice .Methods Xenografts models were established by subcutaneous injection of SW-13 cells into BALB/c nude mice.Tumor-bearing mice were randomly divided into control group （n=8）, intervention group A（n=8） and intervention group B （n=8）,then administrated with normal saline ,0.30 mg/ml HCPT, 0.45 mg/ml HCPT by intraperitoneal injection respectively ,the injection volume was 10 ml/kg,once a day,the administration conducted in the first 5 days and then stopped in the last 2 days every week ,the whole treatment lasted for 2 weeks.The tumor growth curves and event-free survival （ EFS） curves were drawn and measured ,and the optimal %T/C and EFS T/C values of each group were calculated .Results The tumor growth curves of intervention groups were flatter than that of control group,and presented a delay growth .The mean event-free survivals of control group ,intervention group A and intervention group B were 5 days,8 days and 17 days,respectively.The optimal %T/C of intervention group A and intervention group B both were below 40%,which suggested that HCPT had the treatment effects .The EFS T/C value of intervention group A was 1.75,which suggested that 0.30 mg/ml HCPT was of low antitumor effect .The EFS T/C value of intervention group B was 3.25,and the reduction in tumor volume wasn′t observed,which suggested that 0.45 mg/ml HCPT was of intermediate antitumor effect .Conclusion HCPT has antitumor effects against human adrenocortical carcinoma SW-13 xenografts in nude mice ,and it can inhibit the tumor growth .However ,the antitumor effect of the dose needs a further study .

关 键 词：肾上腺皮质癌 羟喜树碱 裸鼠 移植瘤 抗瘤活性 

分 类 号：R736.6[医药卫生—肿瘤]