转Bt基因糙米中外源蛋白质和外源基因在生长猪体内的残留及对组织器官的损伤  

Residues and Organ Damage of Exogenous Gene and Protein from Transgenic Bt Brown Rice in Growing Pigs

作　　者：曹正辉[1,2] 王占彬[2] 顾宪红[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,动物营养学国家重点实验室,北京100193 [2]河南科技大学动物科技学院,洛阳471003

出　　处：《动物营养学报》2014年第7期1908-1915,共8页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：转基因生物新品种培育重大专项课题(2012ZX08011001)

摘　　要：本试验旨在研究饲喂转Bt(Bacillus thuringiensis,Bt)基因糙米的生长猪的器官组织及体液中是否有外源蛋白质及外源基因的残留以及其组织器官的生理结构是否受影响。试验选用30头体重为(35.76±2.52)kg的大白生长猪,随机分为对照组和试验组,每组15头。对照组饲喂基础饲粮+70%非转基因糙米;试验组饲喂基础饲粮+70%转Bt基因糙米。试验期90 d。结果表明:饲粮中添加转Bt基因糙米的生长猪组织器官的亚显微结构没有发生明显病理变化;通过聚合酶链式反应(PCR)和酶联免疫吸附试验(ELISA)方法也未在生长猪的组织器官、体液及排泄物中检测出外源蛋白质和外源基因的残留。结果提示,转Bt基因糙米以70%的比例添加到饲粮中饲喂生长猪,糙米中的外源基因及表达的外源蛋白质通过动物的消化道会被完全地分解消化,没有在动物体内残留,也未损伤组织器官。This experiment was conducted to study the effects of transgenic Bacillus thuringiensis （Bt） brown rice on exogenous protein residues,exogenous gene residues and organ damage in growing pigs.Thirty Large White growing pigs with the body weight of （35.76±2.52） kg were randomly allocated into 2 treatments with 15 replicates per treatment.Pigs in control group were fed a basal diet ＋ 70％ non-transgenic Bt brown rice,and the pigs in test group were fed the basic diet ＋ 70％ transgenic Bt brown rice.The experiment lasted for 90 days.The results showed that dietary supplemented with transgenic Bt brown rice had no effects on the submicroscopic structure of the visceral organs of growing pigs.Exogenous protein and exogenous genes were not found in tissues organs,body fluids and excreta of growing pigs through polymerase chain reaction （PCR） and enzyme-linked immunosorbent assay （ELISA） methods.The results suggest that supplemented 70％ transgenic Bt brown rice in growing pig＇ s diet,the exogenous genes and protein can be completely digested and absorbed by digestive tract without any residual in the body and will not damage the visceral organs.

关 键 词：BT基因 糙米 生长猪 残留 

分 类 号：S828[农业科学—畜牧学]