膜攻击复合物C5b-9在大鼠肝脏缺血再灌注损伤中的表达  

Expression of the membrane attack complex C5b-9 on total hepatic ischemia-reperfusion injury in rats

作　　者：饶艳[1,2] 邓少剑[1] 何志恩 柯剑娟[3] 王焱林[3] 陈永权[4] 

机构地区：[1]广州市番禺区中医院麻醉科,511400 [2]广州市中山大学中山眼科中心 [3]武汉大学中南医院麻醉科 [4]安徽省芜湖市皖南医学院附属弋矶山医院麻醉科

出　　处：《中华临床医师杂志（电子版）》2014年第12期107-109,共3页Chinese Journal of Clinicians(Electronic Edition)

基　　金：番禺区科技计划项目(2012-Z-03-01);安徽省高校省级自然研究项目(KJ2013Z341)

摘　　要：目的：探讨膜攻击复合物C5b-9在大鼠肝脏缺血再灌注损伤中的表达。方法健康雄性SD大鼠60只，180～200 g，随机分为5组（n＝12）：假手术组（S组）、全肝再灌组1 h组（I/R 1 h组）、3 h组（I/R 3 h组）、6 h组（I/R 6 h组）和24 h组（I/R 24 h组）。制备大鼠全肝缺血再灌注模型，再灌注1、3、6、24 h末，取血测定谷丙转氨酶（ALT）和总胆红素（TBIL）浓度。取肝脏组织，检测C5b-9 mRNA和C5b-9蛋白的表达。结果与对照组比较，I/R各组大鼠ALT和TBIL水平均明显升高，I/R 3 h组达到高峰（P＜0.05）；I/R各组C5b-9的mRNA表达和C5b-9蛋白含量均有显著升高，且随着缺血再灌注时间的延长而逐渐升高，其中I/R 24 h组最高（P＜0.05）。结论补体C5b-9参与肝缺血再灌注的损伤，尤其是在再灌注24 h时达到高峰。ObjectiveTo investigate the expression of the membrane attack complex C5b-9 on total hepatic ischemia-reperfusion injury in rats.MethodsSixty male healthy SD rats weighing 180-200 g were randomly divided into 5 groups： control group （S group） and 1 h, 3 h, 6 h and 24 h hepatic ischemia-reperfusion group（I/R 1 h, 3 h, 6 h and 24 h,n=12）. The hepatic I/R model was reproduced, and the concentration of alanine aminotransferase （ALT） and total bilirubin （TBIL） in blood was determined respectively in end of reperfusion for 1, 3, 6, 24 h; Hepatic tissues were obtained and the expression of C5b-9 gene and C5b-9 protein involved in all groups were detected.ResultsCompared with control group, the concentration of ALT and TBIL was increased significantly in all I/R groups （P〈0.05）, which was increased to the peak in I/R 3 h group. The expression of C5b-9 mRNA and protein detected in all I/R groups were higher than those of the control group （P〈0.05） and were peaked in I/R 24 h group. ConclusionActivation of C5b-9 was one of the mechanisms in hepatic ischemia reperfusion injury, especially in the reperfusion for 24 hours.

关 键 词：肝 再灌注损伤 补体膜攻击复合物 

分 类 号：R744.51[医药卫生—神经病学与精神病学]