大肠杆菌O157显色培养基检测效果的评价  

Evaluation of the detecting effect for chromogenic Escherichia coli O157 medium

作　　者：卢勉飞[1] 蔡芷荷[1] 陈霖熙 邱国建[1] 吴清平[2] 杨宁[1] 

机构地区：[1]广东环凯微生物科技有限公司,广东广州510663 [2]广东省微生物研究所,广东广州510070

出　　处：《微生物学通报》2014年第7期1456-1462,共7页Microbiology China

基　　金：广东省重大科技专项项目(No.2012A080203014);广州市科技计划项目(No.201300000074)

摘　　要：【目的】评价显色培养基对大肠杆菌O157:H7(Escherichia coli O157:H7)的检测效果。【方法】本实验室研制的大肠杆菌O157显色培养基(HKM),与国外梅理埃、科玛嘉及国内厂家的同类产品及传统培养基CT-SMAC作比较,对相关菌株以及污染样品和实际样品进行对比测试。【结果】实验室研制的HKM大肠杆菌O157显色培养基与科玛嘉同类产品在特异性、灵敏度及检测效果方面均无明显差异,均优于梅里埃、国内厂家产品及CT-SMAC。【结论】HKM大肠杆菌O157显色培养基具有高检出率及高特异性的特点,具有较好的应用价值和前景。[Objective] To evaluate the efficacy of chromogenic medium for Escherichia coli O157：H7. [Methods] A new Escherichia coli O157 chromogenic medium （HKM） was compared with CHROMagar, BioMerieux, Domestic company and CT-SMAC for detection of Escherichia coli O157 by reference strains, artificially contaminated samples and natural samples. [Results] The selectivity, highly specificity and detection efficiency of HKM were as same as that of CHROMagar, and were better than that of BioMerieux, Domestic company and CT-SMAC. [Conclusion] The HKM E. coli O157 chromogenic medium has a good effect and invaluable in detecting E. coli O157：H7, because it has high specificity and selectivity.

关 键 词：显色培养基 大肠杆菌O157 检测效果 

分 类 号：R446.5[医药卫生—诊断学]