乳糖酶纳米粒的制备及水解牛乳中乳糖的研究  

Preparation of lactase-loaded nanoparticles and investigation of hydrolysis of lactose in milk

作　　者：盛燕[1] 贾静霞[1] 

机构地区：[1]烟台大学化学化工学院,烟台264005

出　　处：《食品科技》2014年第7期2-5,共4页Food Science and Technology

基　　金：国家自然科学基金项目(31300789);山东省自然科学基金项目(ZR2013CQ016)

摘　　要：以具有良好生物相容性和生物降解性的聚乳酸作为壳材，采用改进的5步复乳法制备载乳糖酶纳米粒。对其外观形貌、粒径、包封率和悬浮稳定性等理化性质进行了评价，进一步考察了乳糖酶纳米粒在人工肠液（含胰酶）中的稳定性和水解牛乳中乳糖的效率。结果表明，制备的纳米粒粒径大小均匀（100～200nm）、包封率高（〉85％）且悬浮稳定性好。乳糖酶纳米粒在人工肠液中3h，其活性保留可达（75．63±2．51）％，而对照组游离乳糖酶则几乎完全失活。乳糖酶纳米粒显示了良好的水解乳糖的能力，37℃条件下水解3h，乳糖水解率可达100％，有望成为解决乳糖不耐受症的有效制剂。Using the biocompatible and biodegradable polylactic acid as the particle shell, lactase-loaded nanoparticles were fabricated by a modified five-step double emulsion method in this study. Firstly, the physicochemical characteristics of nanoparticles, such as morphology, particle size, encapsulation efficiency and suspension stability were evaluated. The stability and lactose hydrolysis of lactase-loaded nanoparticles incubated in the pancreatin-containing simulated intestinal fluid were further evaluated, respectively. The results showed that nanoparticles with uniform particle size （100-200 nm）, high encapsulation efficiency （〉85%） as well as high suspension stability could be obtained. The prepared lactase-loaded nanoparticles could retain （75.63±2.51）% of its activity after 3 h incubation in the simulated intestinal fluid, whereas the control free lactase was almost completely degraded under the same condition. Lactase-loaded nanoparticles exhibited strong ability to hydrolyze lactose in milk, reaching nearly 100% of hydrolysis ratio at 37 ℃ for 3 h. These results suggest that these lactase-loaded nanoparticles represent promising candidates for efficient management of lactose intolerance.

关 键 词：乳糖酶 纳米粒 乳糖水解 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]