超声波法优化提取双孢蘑菇废弃菇柄蛋白质  

Optimize ultrasonic extraction of protein from Agaricus bisporus waste stipe

作　　者：李顺峰[1] 王安建[1] 侯传伟[1] 沈琪[1] 彭玲[1] 魏书信[1] 

机构地区：[1]河南省农科院农副产品加工研究所,郑州450002

出　　处：《食品科技》2014年第7期258-261,共4页Food Science and Technology

基　　金：河南省重大科技攻关计划项目(112101110300)

摘　　要：为促进双孢蘑菇废弃菇柄的综合利用,增加其经济价值,以双孢蘑菇废弃菇柄为试材,采用超声波提取菇柄中的蛋白质。在单因素试验基础上,选取超声波功率、液固比、pH值为影响因素,以蛋白质提取率为指标,采用正交试验对蛋白质提取进行优化。结果表明,在超声处理时间为1 min时,蛋白质最佳提取工艺参数为超声波功率150 W、液固比35:1 mL/g、pH10.0,在此优化条件下蛋白质提取率可达6.87 g/100 g。与传统的碱法提取相比,超声波法蛋白质提取率约为碱法提取的3.5倍,时间缩短了60倍。超声波提取操作简单、高效、快速、安全,反应过程无副反应发生,是一种安全、经济和简便的提取方法,可用于多种有效物质提取。To promote the utilization of Agaricus bisporus waste stipe and increase its economic value, protein were extracted from Agaricus bisporus waste stipe by ultrasonic extraction. The effect of ultrasonic power, ratio of liquid to solid, ultrasonic time, pH value on the yield of protein were investigated by single factor experiments, and optimized the parameters by orthogonal experiment use ultrasonic power, ratio of liquid to solid and pH value as factors. The results showed that as the ultrasonic time was 1 min, the parameters optimized by orthogonal experiment analysis were as follows： ultrasonic power 150 W, liquid-solid ratio 35：1 mL/g, pH value 10.0. Under these optimum conditions, the yield of protein extract from Agaricus bisporus waste stipe can be up to 6.87 g/100 g, which was 3.5 times higher than traditional alkali extraction, and shorten 60 times than traditional alkali extraction in time. As a simple, effective, fast, safe and no adverse reaction, ultrasonic extraction is effective extraction method, and can be used to extract all functional components.

关 键 词：双孢蘑菇 废弃菇柄 蛋白质 超声波提取 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程] TS255.3[轻工技术与工程—食品科学与工程]