急性羰基镍中毒大鼠肝脏中ATP酶活力及其基因表达的实验观察  

Experimental observation on ATP enzyme activity and its gene expression in rat liver of acute nickel carbonyl poisoning

作　　者：王辉[1,2] 王宁[1] 王秋英 蒲宏全 罗琴[1] 尚慧 马海霞[1] 马国煜 程宁[1] 

机构地区：[1]兰州大学甘肃省新药临床前研究重点实验室,甘肃兰州730000 [2]兰州大学公共卫生学院流行病与卫生统计学研究所,甘肃兰州730000 [3]金川公司职工医院,甘肃金昌737103

出　　处：《工业卫生与职业病》2014年第4期247-250,254,共5页Industrial Health and Occupational Diseases

摘　　要：目的通过研究大鼠急性羰基镍中毒肝脏中钠钾ATP酶(Na+-K+ATPase)活力变化与钠钾ATP酶α1(Na+-K+ATPaseα1)基因表达,探讨急性羰基镍中毒对肝脏的急性毒性作用及其中毒机制。方法健康SPF级SD大鼠200只,分为5组,雌雄各半,静态吸入染毒,染毒时间为30min,羰基镍染毒浓度分别为20、135和250mg/m3,氯气染毒250mg/m3为阳性对照组,健康大鼠为正常对照组,染毒后第1、第2、第3和第7天分别取材,用定磷比色法测定Na+-K+ATP酶活力,用反转录-聚合酶链反应(RTPCR)方法测定Na+-K+ATPα1基因的表达水平。结果在低、中、高浓度羰基镍染毒条件下,肝脏中Na+-K+ATP酶活力均明显下降(P<0.05),以染毒第3天时肝脏中Na+-K+ATP酶降低最为明显(P<0.05);高浓度染毒组染毒第3天,肝脏中Na+-K+ATPα1基因表达低于对照组(P<0.05)。结论羰基镍可引起大鼠肝脏氧化损伤,明显降低Na+-K+ATP酶活力并影响α1基因表达。Objective By studying dynamic change of Na＋-K＋ ATPase and gene expression of Na＋-AT Pase a 1 in acutely nickel carbonyl poisoned rat livers to explore its acute toxicity in liver function and its intox- ication mechanism, Methods 200 healthy SPF SD rats half-and-half in male and female were divided into 5 groups. Three exposed groups composed of statically inhaling nickel carbonyl of 20, 135 and 250 mg/m3 re- spectively for 30 rain. Chlorine exposure to 250 mg/m3 was taken as the positive control group. Healthy rats were taken as the normal control group. All animals were sacrificed and sampled at the 1st, 2nd, 3rd, and 7th d respectively. Colorimetric phosphorus determination method was used to determine the activity of Na＋-K＋ AT- Pase, reverse transcription-polymerase chain reaction （RT-PCR） method was used to determine gene expres- sion level of Na＋-K＋ATP a 1. Results In all three dosage exposed groups, the activities of Na＋-K＋ ATPase in liver significantly lowered （P〈0. 05）, that of the 34 d appeared the most obvious （P〈0. 05）. In high dos- age exposed group the gene expression of Na＋-K＋ ATP a 1 in liver at the 3rd d showed lower than that of the control group （P〈0. 05）. Conclusions The carbonyl nickel could significantly induce oxidative damage in liver tissue to lower the activity of Na＋-K＋ ATPase affecting the gene expression of α 1.

关 键 词：羰基镍 急性毒性 Na+-K+ATP酶 Na+-K+ATPase A1基因 

分 类 号：R114[医药卫生—卫生毒理学]