华南地区肉鸽源沙门氏菌的鉴定及遗传进化分析  

Identification and Genetic Evolution Analysis of Salmonella Isolated from Pigeon in South China

作　　者：李雪雁[1,2] 付涛[1] 纪桂云[1] 张津津[1] 江奕容 林晓霜[1] 

机构地区：[1]电子科技大学中山学院,广东中山528402 [2]中山大学生命科学学院,广东广州510275

出　　处：《中国畜牧兽医》2014年第7期207-211,共5页China Animal Husbandry & Veterinary Medicine

基　　金：中山市科技计划项目(20114A207)

摘　　要：本试验从华南地区发病肉鸽肠道和肝脏中用BS培养基分离、纯化沙门氏菌,并进行多项生化试验和血清型鉴定试验,结果显示各项生化检测指标均符合沙门氏菌的特点;血清型鉴定试验结果表明该分离菌属沙门氏菌属A-F群。进一步对该分离菌的16SrRNA片段测序及遗传进化分析,确定该分离菌为沙门氏菌菌株,与鼠伤寒沙门氏菌同源性为100%。对该分离菌进行动物回归试验,结果表明该沙门氏菌菌株为华南地区肉鸽的致病菌株。该结果为进一步研究华南地区肉鸽沙门氏菌的致病机理和防控奠定了基础。Salmonella was isolated using BS selective medium from liver and intestinal contents of diseased pigeon raising inSouth China and made the purified culture, biochemical test, serotype experiment, sequencing analysis of 16S rRNA and re-gression test. A single colony of Salmonella was isolated, identified by biochemical experiments. Serotype experiment resultcombined with the biochemical testing results were showed that the strain was the result of A polyvalent F. After aligning thesequence of 16S rRNA fragments with the sequences in GenBank, the results of genetic evolution analysis showed that the simi-larity of pigeon Salmonella and Salmonella paratyphi was 100%. In the animal regression test,it showed that the Salmonellaisolated in pigeon was pathopoiesis.

关 键 词：肉鸽 沙门氏菌 分离鉴定 16SrRNA 遗传进化分析 

分 类 号：S831.2[农业科学—畜牧学]