山东省肉鸭沙门氏菌的分离鉴定及敏感抗菌药物的筛选  

Isolation and Identification of Duck Salmonellaand Screening of Sensitive Antimicrobials in Shandong Province

作　　者：齐静[1] 王守荣[2] 李兰波[3,4] 骆延波[1] 李璐璐[1] 胡明[1] 王进文[1] 李虹达[1] 贾纪美 赵敏[1] 杨胜福[1] 李玉保[2] 单虎[4] 刘玉庆[1,4] 

机构地区：[1]山东省农业科学院畜牧兽医研究所,山东省畜禽疫病防治与繁育重点实验室,山东济南250100 [2]聊城大学农学院,山东聊城252059 [3]山东省医学科学院,山东省实验动物中心,山东济南250002 [4]青岛农业大学,山东省兽药诊断试剂工程技术研究中心,山东省预防兽医学重点实验室,山东青岛266109

出　　处：《中国畜牧兽医》2014年第7期216-220,共5页China Animal Husbandry & Veterinary Medicine

基　　金：国家科技重大专项子课题(2013ZX10004217003001);科技基础性工作专项(2012FY111000);山东省自然科学基金(ZR2013CQ037);山东省科技发展计划(2012GGC02012、2013GNC11032);山东省现代农业产业技术体系建设经费(SDAIT-13-011-09);山东省农业重大应用技术创新课题“超耐药菌在食物链中流行病学监测与防控技术研究”

摘　　要：从山东省各个地区的病死肉鸭采集317份组织样品进行细菌分离,并进行染色镜检和生化鉴定,共分离鉴定出105株沙门氏菌,分离率为33.1%。将临床推荐用药浓度的0.1倍作为抗药性检测浓度L,设置不同药物浓度梯度(1/4L、1/2L、L、2L和4L),进行17种抗生素的药敏试验,根据L药物浓度梯度对分离菌株的抑菌率大于80%的原则进行敏感抗菌药物的筛选。结果表明,沙门氏菌是危害山东省肉鸭养殖的主要病原菌之一,且多重耐药现象较严重,其中氨苄西林、诺氟沙星、卡那霉素、氟苯尼考和培氟沙星等敏感药物可作为临床推荐药物对沙门氏菌进行防控。317 tissue samples of sick and dead ducks from various regions of Shandong province were collected for bacterialisolation, staining microscopy and biochemical identification. Totally, 105 strains of Salmonella were identified and the separa-tion rate was 33.1%. Antimicrobial susceptibility testing of all isolates to 17 kinds of antibiotics was carried out by setting updifferent concentration gradient （1/4 L, 1/2 L, L, 2 L and 4 L） of the clinical recommended dose. The sensitive antimicrobialsto the isolates were screened according to the inhibition ratio greater than 80% at the concentration of L gradient. The resultshowed that Salmonella was one of the main pathogenic bacteria in duck breeding of Shandong province, and with severe multi-ple drug resistance. Several sensitive antimicrobials including ampicillin, norfloxacin, kanamycin, florfenicol and pefloxacincould be used as clinical recommended drugs for the prevention and control of this disease.

关 键 词：沙门氏菌 分离 鉴定 药敏试验 鸭 

分 类 号：S852.61[农业科学—基础兽医学]