EphB4和VEGF在食管癌中表达与其胸腔镜下根治术疗效的相关性研究  

Expression of EphB4 and VEGF in esophageal cancer tissues and their relationship with microvessel density and the curative effect under thoracoscope

作　　者：张明灿 游凯 黄一 张明静[2] 陈虎平 裴家强 龚文清 游振飞 

机构地区：[1]解放军三二四医院心胸外科,重庆400000 [2]解放军后勤工程学院医院门诊部,重庆400000

出　　处：《重庆医学》2014年第20期2600-2602,共3页Chongqing medicine

摘　　要：目的探讨促红细胞生成素的肝细胞癌B4受体(EphB4)、血管内皮生长因子(VEGF)在食管癌中的表达及其与血管密度的关系,并分析行胸腔镜下根治术的疗效。方法采用免疫组织化学法检测76例食管癌和癌旁组织中EphB4、VEGF的表达,使用微血管密度(MVD)值(免疫组织化学CD34染色判断)判断组织的血管密度,比较之间的关系。根据EphB4、VEGF表达情况分析其与患者在行胸腔镜下根治术淋巴结转移率、复发率和5年生存率的关系。结果 EphB4、VEGF在癌组织的阳性表达率分别为57.89%、61.84%均显著高于癌旁组织的0和7.89%(P<0.05)。MVD高的患者EphB4、VEGF阳性表达率分别为67.44%和76.19%,显著高于MVD低的患者45.45%和44.11%(P<0.05)。伴有淋巴结转移组和伴有复发组的EphB4、VEGF表达阳性率均显著高于无淋巴结转移组和无复发组的(P<0.05);且大于或等于5年生存率的患者中EphB4、VEGF表达阳性率分别为45.00%和45.45%,显著低于小于5年生存率的患者80.36%和85.19%,差异有统计学意义(P<0.05)。结论 EphB4和VEGF在食管癌中高表达,与血管密度密切相关,与淋巴结转移、复发及患者5年生存率有关;EphB4和VEGF阳性表达者术后疗效较差。Objective To explore the expression of EphB4 and VEGF in esophageal cancer tissues and their relationship with microvessel density （MVD ） ,and analysis the curative effect of postoperative esophageal cancer radical under thoracoscope . Methods Theexpression of EphB4 and VEGF was detected by immunohistochemistry in tumor specimens from 76 cases of esopha-geal squamous cell carcinoma and paratumor normal specimens ,used CD34 as marker to count MVD .According to the situation of expression of EphB4 and VEGF ,we analysis their relationship with lymph node metastasis rate ,recurrence and 5-year survival rate . Results The positive expression rate of EphB4 and VEGF in cancerous tissue （57 .89% and 61 .84% ） ,were significantly higher than that in tissue adjacent to carcinoma（0 and 7 .89% ）（P〈0 .05） .The positive expression rate ofEphB4 and VEGF in high MVD values of patients （67 .44% and 76 .19% ） ,were significantly higher than thatin low MVD values of patients （45 .45% and 44 .11% ）（P〈0 .05） .The positive expression rate ofEphB4 and VEGF in the patientswith lymph node metastasis group and associ-ated with recurrence ,were significantly higher than that of group without lymph node metastasis and group without recurrence （P〈0 .05） .The positive expression rate of EphB4 and VEGF in patients of greater than or equal to 5 years of survival rate（45 .00% and 45 .45% ） ,were significantly lower than in patientsof Less than 5 years of survival rate （80 .36% and 85 .19% ）（P〈0 .05） .Conclu-sion EphB4 and VEGF are highly expressed in esophageal cancer tissue ,which may be closely associated withmicrovessel density , and lymph node metastasis ,recurrence and 5 years survival rate ;the curative effect of positive expression rate of EphB 4 and VEGF is poor .

关 键 词：血管内皮生长因子 食管肿瘤 肝细胞癌B4受体 血管密度 

分 类 号：R735.1[医药卫生—肿瘤]