细梗香草皂甙对鼻咽癌CNE-2细胞的体外抗瘤作用  

The Antitumor Effect of Capilliposide on Nasopharyngeal Cancer Cell Line CNE-2 in vitro

作　　者：花永虹[1] 胡巧英[1] 朴永锋[1] 唐秋[1] 马胜林[2] 

机构地区：[1]浙江省肿瘤医院,浙江杭州310022 [2]杭州市第一人民医院,浙江杭州310006

出　　处：《中国肿瘤》2014年第7期597-600,共4页China Cancer

摘　　要：[目的]观察细梗香草皂甙抗人鼻咽癌CNE-2细胞的效应。[方法]细梗香草皂甙与CNE-2细胞共培养,采用MTT法检测细胞增殖,软琼脂实验检测细胞集落形成情况,流式细胞仪检测细胞凋亡。[结果]不同浓度细梗香草皂甙作用于CNE-2细胞后,细胞存活率显著性降低,48h细梗香草皂甙IC50为7.4μg/ml。CNE-2细胞经8μg/ml细梗香草皂甙处理后,软琼脂集落形成能力下降,集落形成数为119±11个,比对照组(297±24个)低;细梗香草皂甙组的凋亡率达16.43%±3.1%,而对照组为9.34%±2.3%。[结论]细梗香草皂甙对鼻咽癌CNE-2细胞株有抑制增殖和诱导凋亡的作用。[Purpose] To observe the antitumor activity of capilliposide on human nasopharyngeal cancer cell lines CNE-2. [Methods] CNE-2 cells were cultured with different concentrations with capilliposide. Cell proliferation were measured by M＇I＇T assay,colony formation by a soft agar assay and apoptosis by flow cytometry. [Results] The survival rate of CNE-2 cells was significantly de- creased after incubation with capilliposide,and the IC50 in 48 hours was 7.4μg/ml. After 8μg/ml concentrations treatment,the number of colony-formation in soft agar of the CNE-2 cells was 297± 24,which was lower than that in control group（119±11）. Apoptosis rate was 16.43%±3.1% in capil- liposide group and 9.34%±2.3% in control group. [Conclusion] Capilliposide plays a role in growth inhibition and inducing apoptosis in human nasopharyngeal cancer cell line CNE-2.

关 键 词：鼻咽肿瘤 细梗香草皂甙 细胞增殖 凋亡 

分 类 号：R739.63[医药卫生—肿瘤]