高脂高糖饮食对家兔糖耐量影响实验研究  

High fat-sucrose diet effect on glucose tolerance of rabbits

作　　者：张光伟[1] 林燕[2] 朱宁红[2] 白艳萍[2] 杨佩刚[2] 刘恩岐[2] 

机构地区：[1]西安医学院临床学院 [2]西安交通大学医学院实验动物中心,西安710061

出　　处：《陕西医学杂志》2014年第7期771-773,778,共4页Shaanxi Medical Journal

基　　金：国家自然科学基金项目(81070250);陕西省公共平台建设基金项目(2010FWPT-15)

摘　　要：目的:探讨高脂高糖饮食对日本大耳白兔糖代谢和脂代谢的影响,建立日本大耳白兔代谢综合征模型。方法:将20只雄性日本大耳白兔随机分为两组(每组10只),对照组饲喂基础饲料,实验组饲喂高糖高脂饲料(配方:10%猪油+30%蔗糖+60%基础饲料),观察36周,期间每2周抽血1次,检测血糖和甘油三酯的水平。于第36周结束时,对动物进行葡萄糖耐量试验,而后麻醉处死动物,观察动物重要脏器和全身脂肪重量变化。结果:实验组家兔在36周时,体重[(3.90±0.09)kg vs(3.18±0.27)kg]、脂肪重量[总脂肪:(493.40±55.25)g vs(331.45±38.28)g]和内脏脂肪[(354.90±48.18)g vs(214.35±23.74)g]均显著高于对照组,实验组和对照组之间比较存在显著性差异。结论:高糖高脂可诱导日本大耳白兔引起糖代谢紊乱和中心性肥胖,这为代谢综合征的研究提供实验和理论基础。Objective ：To induce metabolic syndrome in Japanese white rabbit by high fat/high sucrose diet （HFSD） .Methods：Twenty male Japanese white rabbits were divided into 2 groups by randomized block de-sign ：normal diet group （n=10） and HFSD group （n=10 ,with 10 % lard ,30 % white cane sugar and 60 % nor-mal fodder） ,and observed for 36 weeks .The blood samples of the rabbits were tested for the levels of plasma glu-cose and triglyceride after overnight fasting every two weeks .At the end of 36th week ,after intravenous glucose tol-erance test （IVGTT） ,all the rabbits were killed to observe vital organs and weight all adipose tissues .Results：The difference between the 2 groups was not significant in plasma glucose ,triglyceride and insulin except in IVGTT . HFSD group were higher in body weight [（3 .90 ± 0 .09） kg vs （3 .18 ± 0 .27） kg] and adipose tissue [total adipose tissue：（493 .40 ± 55 .25） g vs （331 .45 ± 38 .28） g ;visceral adipose tissue ：（354 .90 ± 48 .18） g vs （214 .35 ± 23 .74） g] as compared with control group ,and the difference was significant .Conclusion：HFSD can induce impaired glu-cose tolerance and trend of central obesity in Japanese white rabbits .

关 键 词：肥胖症 病理生理学 葡萄糖代谢障碍 代谢 模型  动物 葡糖耐量试验 家兔 

分 类 号：R363[医药卫生—病理学]