表没食子儿茶素没食子酸酯对柔红霉素致小鼠心脏毒性的预防作用  

Protective Effects of(-)-Epigallocatechin Gallate on Daunorubicin-induced Cardiotoxicity in Mice

作　　者：李丽[1,2] 唐燕霞[1] 黄志明[1] 陈润丽[1] 周焕第[2] 刘布鸣 梁钢[1] 

机构地区：[1]广西医科大学药学院,南宁530021 [2]广西中医药大学药学院,南宁530001 [3]广西中药质量标准研究重点实验室,南宁530022

出　　处：《医药导报》2014年第7期841-844,共4页Herald of Medicine

基　　金：国家自然科学基金资助项目(81160532);教育部博士点基金博导类课题(20134503110007);广西自然科学基金资助项目(2012GXNSFAA053147);广西中药质量标准研究重点实验室(广西壮族自治区中医药研究院)开放课题基金项目(桂中重开201105)

摘　　要：目的观察表没食子儿茶素没食子酸酯(EGCG)对柔红霉素(DNR)所致小鼠心脏毒性的预防作用。方法将合格小鼠随机分为4组,即正常对照组、模型对照组(DNR致心脏毒性组)、EGCG高剂量组(80 mg·kg-1)、EGCG低剂量组(40 mg·kg-1)。给予EGCG 7 d后,除正常对照组外,其余各组小鼠腹腔注射DNR(15 mg·kg-1)。48 h后检测心电图、血清心肌酶谱、高敏肌钙蛋白T(TNT-Hs)、心肌超微结构。结果模型对照组小鼠心律失常发生率64.7%,丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、肌酸激酶(CK)、肌酸激酶MB同工酶(CK-MB)、乳酸脱氢酶(LDH)、α-羟基丁酸脱氢酶(α-HBDH)和TNT-Hs显著高于正常对照组(P<0.01),心肌超微结构明显损伤;EGCG高剂量组小鼠心律失常发生率44.4%,EGCG低剂量组小鼠心律失常发生率31.6%;EGCG各剂量组小鼠血淸心肌酶及TNTHs较模型对照组显著降低(P<0.05或P<0.01);EGCG低剂量组小鼠心肌超微结构损伤较模型对照组明显减轻。结论EGCG对DNR所致小鼠心脏毒性有一定的预防作用。Objective To investigate the protective effects of （-）-epigallocatechin gallate （ EGCG ） on daunorubicin （ DNR）-induced cardiotoxicity in mice. Methods The qualified mice were randomly divided into four groups：normal control group, myocardial injured model control group,high dose group （80 mg·kg-1 ） and low dose group （40 mg·kg-1 ） of EGCG. EGCG was administered intragastrically once daily for 7 days,followed by a single intraperitoneal injection of DNR （15 mg·kg-1 ） except in the normal control group. The electrocardiogram,myocardial enzymes and TNT-Hs in serum,cardiac ultrastructure of mice were detected after 48 h. Results In DNR model control group,the incidence of arrhythmia was 64. 7%. The activity of serum cardic enzymes including CK,CK-MB,LDH,α-HBDH and ALT,AST, level of TNT-Hs were significantly higher than those in the normal control group（P〈0. 01）,and myocardial ultrastructure was injured remarkably. The incidence of arrhythmia was 44. 4% in mice treated with high dose of EGCG and 31. 6% in mice with low dose of EGCG. Compared to the model control group, the activity of CK,CK-MB,LDH,α-HBDH and ALT,AST, level of TNT-Hs in serum decreased remarkably in EGCG groups（ P〈0. 05 or P〈0. 01）. Low can EGCG alleviated the injury to the ultrastructure of myocardium compared to the model control group. Conclusion EGCG can prevent the cardiac toxicity induced by DNR in mice.

关 键 词：表没食子儿茶素没食子酸酯 柔红霉素 心脏毒性 心肌酶 

分 类 号：R286[医药卫生—中药学] R965[医药卫生—中医学]