牛瑟氏泰勒虫p23-IL-18融合基因的原核表达  

Prokaryotic Expression of the p23-IL-18 Fusion Gene of Theileria sergenti

作　　者：白杨[1] 许应天[2] 吴艳丽[1] 于志云[1] 

机构地区：[1]吉林正业生物制品股份有限公司,吉林吉林132101 [2]延边大学,吉林延吉133002

出　　处：《中国兽药杂志》2014年第7期6-10,共5页Chinese Journal of Veterinary Drug

摘　　要：为探索牛瑟氏泰勒虫病基因工程亚单位疫苗的可行性,以p23-IL-18融合基因克隆到原核表达载体pET-28a,构建原核重组质粒pET-28a-p23-IL-18,用IPTG诱导表达,对表达产物进行SDS-PAGE和Western-blotting分析。结果表明,构建牛瑟氏泰勒虫pET-28a-p23-IL-18原核表达质粒,目的基因在大肠杆菌中获得表达,融合蛋白的分子量约为48 kDa,并被牛瑟氏泰勒虫阳性血清所识别,具有良好的反应原性。此结果为牛瑟氏泰勒虫病基因工程亚单位疫苗的制备提供了理论依据。In order to prepare the genetically engineering subunit vaccine of brovine Theileria sergenti, the p23-IL-18 fusion gene was cloned into prokaryotic pET-28a expression vector in this experiment. It can construct recombinant prokaryotic expression plasmid pET-28a-p23-IL-18 which induced expression by IPTG. The expression products were examined by SDS-PAGE and Western-blotting. The results showed that the prokaryotic expression plasmid pET-28a-p23-IL-18 was built successfully and the objective gene was successfully expressed in Escherichia coli. The molecular weight of fusion protein was 48 kDa and could react with Theileria sergenti positive serum with good antigencity. The results of this research laid the foundations for genetically engineering subunit vaccine of brovine Theileria sergenti.

关 键 词：牛瑟氏泰勒虫 p23-IL-18融合基因 原核表达 

分 类 号：S852.72[农业科学—基础兽医学]