解几丁质类芽胞杆菌几丁质酶活性及其次生代谢物的测定  

The Caracteristics of Citinase Ativity and Scondary Mtabolites in Paenibacillus Citinolyticus

作　　者：郑梅霞[1] 陈峥[1] 刘波[1] 刘程程[1,2] 车建美[1] 蓝江林[1] 潘志针[1] 朱育菁[1] 唐建阳[1] 

机构地区：[1]福建省农业科学院农业生物资源研究所,福建福州350003 [2]福建农林大学生命科学院,福建福州350002

出　　处：《福建农业学报》2014年第5期492-497,共6页Fujian Journal of Agricultural Sciences

基　　金：科技部973前期项目(2011CBl11607);农业部948项目(2011-G25);福建省财政专项--福建省农业科学院科技创新团队建设基金(STIF-Y03);国家自然科学基金(31370059);福建省农业现代化发展战略研究项目(ZDZX-1301);科技部国际合作项目(2012DFA31120)

摘　　要：采用几丁质酶培养基筛选出FJAT-10038芽胞杆菌,测定其几丁质酶活力为11.04U·mL-1,说明FJAT-10038芽胞杆菌能够产几丁质酶。采用气相色谱质谱联用(GC/MS)的方法分析FJAT-10038发酵液丙酮萃取的成分,初步鉴定出31种化合物。在鉴定出的化合物中,相对含量超过10%的有2种,分别是六氢化-吡咯环[1,2-a]-吡嗪-1,4-二酮和异丁醚,相对含量分别为44.17%和15.58%;匹配率在90%以上的成分有3种,分别是二叔丁基对甲酚、六氢化-吡咯环[1,2-a]-吡嗪-1,4-二酮和六氢-3-苯甲基-吡咯并[1,2-a]-吡嗪-1-4-二酮,匹配度分别是90%、97%和93%,相对含量分别是0.43%、44.17%和0.38%;另外还发现一些其他的活性物质。The chitinase activity of Paenibacillus chitinolyticus FJAT-10038 was characterized by filtering FJAT-10038 Bacillus using chitinase medium ,the chitinase activity of which reached at 11.044 U mL ^-1 .Furthermore , Acetone extracts of FJAT-10038 Bacillus were analyzed by GC-MS . Our results suggest that thirteen-one compounds are identified ,and the content of Pyrrolo [1 ,2-a] pyrazine-1 ,4-dione ,hexahydro- and Propane ,1-ethoxy-2-methyl-reached 44.1672% and 15.5814% .Meanwhile ,the match degree of Butylated Hydroxytoluene , Pyrrolo [1 , 2-a ] pyrazine-1 , 4-dione , hexahydro- and Pyrrolo [1 , 2-a ] pyrazine-1 , 4-dione , hexahydro-3-（phenylmethyl）-were 90% ;97% and 93% ,with a relative amount by 0.4305% ,44.1672% and 0.3842% .In addition ,some other active substances were also been characterized .

关 键 词：几丁质酶 气相色谱质谱联用 解几丁质类芽胞杆菌 丙酮萃取 

分 类 号：S646[农业科学—蔬菜学]