NMDA致婴儿痉挛症模型幼鼠血清皮质酮、ACTH及海马CRHmRNA表达的研究  

Effect of N-methyl-D-aspartate-induced infantile spasms on serum corticosterone and adrenocorticotropic hormone levels and hippocampal CRHmRNA expression in young rats

作　　者：王雅洁[1] 邹丽萍[2] 

机构地区：[1]首都医科大学附属北京天坛医院儿科,北京100050 [2]解放军总医院儿科,北京100852

出　　处：《中华神经医学杂志》2014年第7期686-689,共4页Chinese Journal of Neuromedicine

基　　金：国家重点基础研究发展计划（2012CB517903）;国家自然科学基金资助项目（81211140048,30770747,81071036）;北京市自然科学基金资助项目（7081002）;北京首发基金资助项目（2003-2037）;卫生行业专项科研基金资助项目（200802074）;中国博士后科学基金资助项目（201150M1529）

摘　　要：目的研究NMDA致痫对幼鼠血清皮质酮、促肾上腺皮质激素（ACTH）以及海马CRHmRNA表达的影响。方法将P11Wistar幼鼠按随机数字表法分为NMDA致痫组和空白对照组，每组共20只。腹腔注射NMDA和生理盐水后观察3h，观察幼鼠行为学改变并进行癫痫评分；断头取血、分离海马组织，应用放射免疫分析法检测血清皮质酮及ACTH水平，原位杂交方法检测海马组织CRHmRNA表达水平。结果对照组血清皮质酮、ACTH浓度分别为（7．95±0．921ng／mL、（64．17±6．15）pg／mL，NMDA致痫组分别为（13．34±2．18）ng／mL、（115．9±16．8）pg／mL，2组血清皮质酮及ACTH浓度差异有统计学意义（t=10．198，P=0．000；t=12．91，P=0．000）。对照组海马区CRHmRNA阳性表达率为15％（3／201，NMDA致痫组为60％（12／201，2组间差异具有统计学意义萨8．640，P=0．003）。NMDA致痫幼鼠血清皮质酮及ACTH明显增高，海马CRHmRNA阳性细胞增多。结论致痫后海马CRHmRNA表达增加，垂体-肾上腺轴活性明显改变。Objective To study the effect ofN-methyl-D-aspartate （NMDA）-induced seizures on corticosterone and adrenocorticotropic hormone （ACTH） levels and hippocampal CRHmRNA expression in young rats. Methods Wistar rats at P11 were used in our study and randomly divided into NMDA-induced seizure group （n=20） and control group （n=20）; P11 rats of NMDA-induced seizure group received intraperitoneal injection of 7 mg/kg NMDA; normal saline was given to the control group; three h after that, the behavioristics changes were observed, and epilepsy rating was performed. And then, these rats were sacrificed; corticosterone and ACTH levels were determined using radio immunoassay; oligonucleotide CRH mRNA probe was labeled with in situ hybridization. Results The levels of corticosterone and ACTH in the control group （[7.95±0.92] ng/mL and [64.17±6.15] pg/mL） were obviously higher than those in the NMDA-induced seizure group （[ 13.34 ±2.18] ng/mL and [ 115.9± 16.8] pg/mL）, with significant differences （t=l 0.198, P=0.000; t=12.91, P=0.000）. In situ hybridization of CRH mRNA showed that the rate of CRH mRNA-positive cells in the hippocampus of control group was 15% （3/20）, which was significantly lower than that in the NMDA-induced seizure group （60%; X2=8.640, P=0.003）. Conclusion serum corticosterone, ACTH and hippocampal CRH mRNA levels in NMDA-induced seizure rats are significantly increased, indicating that pituitary-adrenal axis is activatedby NMDA-induced seizures in young rats.

关 键 词：婴儿痉挛症 促肾上腺皮质激素释放激素 促肾上腺皮质激素 皮质酮 海马 N-甲基-D天门冬氨酸 

分 类 号：R742.1[医药卫生—神经病学与精神病学]