脑室周围白质软化症早产儿血清microRNA表达谱的初步研究  

MicroRNA expression in premature infant with periventricular leukomalacia

作　　者：吴秋萍[1] 杜江[1] 方素珍[1] 周细中[1] 郑娓[1] 郭海文[1] 邹建群 李宏[1] 

机构地区：[1]南方医科大学珠江医院儿科中心,广州510282

出　　处：《中华神经医学杂志》2014年第7期690-694,共5页Chinese Journal of Neuromedicine

摘　　要：目的通过比较脑室周围白质软化症（PVL）早产儿和健康早产儿血清中microRNA（miRNA）的表达差异，为PVL的早期诊断提供生物学标记物，并为进一步探讨miRNA在PVL发病机制中的作用及探索新的治疗策略提供理论依据。方法选择南方医科大学珠江医院儿科中心自2006年1月至2012年12月收治的7例PVL早产儿和同期5例健康早产儿为研究对象，采集所有对象外周血，提取RNA，应用miRNA芯片筛选miRNA表达谱；采用microcosm、miRanda和targetscan3个miRNA靶基因预测数据库共同富集的结果及G0分析，筛选与神经、血管、遗传发育靶基因相关的miRNA进行实时荧光定量PCR验证。结果PVL早产儿与健康早产儿的miRNA表达谱有显著差异，有100个miRNA表达差异有统计学意义俨〈0．05），其中34个上调，66个下调。用实时荧光定量PCR技术验证10个经上述数据库筛选出的miRNA，其中hsa-miR-323a-3p、hsa—miR．671-3p、hsa．miR．330．3p、hsa—miR-654．5p、hsa．miR-498、hsa—miR-149—3p、hsa．miR．1228．5p共7个miRNA与对照组相比表达差异有统计学意义（P〈0．05），hsa-miR-323a-3p、hsa-miR-671-3p、hsa-miR-330—3p、hsa-miR-654-5p表现为上调，与miRNA芯片结果一致。结论miRNA参与了PVL疾病的发生发展过程。Objective To study the miRNAs differential expressions in premature infants with periventricular leukomalacia （PVL） and healthy premature infants to provide biological markers for early diagnosis of PVL, and to explore the role of miRNAs in PVL to provide theoretical foundation for new treatment strategy. Methods Peripheral blood from 7 premature infants with PVL and 5 healthy premature infants were collected; RNA was harvested using kit. MiRNAs profiling was performed using Exiqon microRCURY LNA miRNAs array. Using the mutual enrichment results from microcosm, miRanda and targetscan and GO analysis, miRNAs from which target genes were associated with nerves, blood vessels and genetic development were screened, and real-time quantitative PCR was, then, employed to validate them. Results The miRNAs expression profiles were significantly different between premature infants with PVL and healthy donors; as compared with healthy donors, 100 miRNAs in the PVL patients were differentially expressed （P〈0.05）, including 34 up-regulated ones and 66 down-regulated ones. Real-time PCR indicated 10 miRNAs; among them, hsa-miR-323a-3p, hsa-miR-671-3p, hsa-miR-330-3p, hsa-miR-654-5p, hsa-miR-498, hsa-miR-149-3p and hsa-miR-1228-5p were differentially expressed （P〈0.05）; AND hsa-miR-323a-3p, hsa-miR-654-5p, hsa-miR-671-3p and hsa-miR-330-3p had increased expressions, enjoying the same trend with miRNA microarray results. Conclusion Parts of miRNAs expression profiles are significantly different in premature infants with PVL, indicating that it may play an important role in pathogenesis of PVL.

关 键 词：白质软化症 早产儿 MICRORNA 

分 类 号：R742[医药卫生—神经病学与精神病学]