H9N2亚型禽流感病毒疫苗候选株的构建  

Construction of H9N2 subtype avian influenza virus vaccine candidate

作　　者：王增[1] 冯华朋[1] 朱鹏阳[1] 邬丽[1] 曾显营[1] 邓国华[1] 施建忠[1] 陈化兰[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2014年第7期571-573,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家重大科技专项(2012ZX10004214)

摘　　要：为研制H9N2亚型禽流感病毒疫苗,本研究以具有良好抗原性的代表病毒株A/chicken/Hunan/S933/2008(H9N2)(HuN33)为HA和NA基因的供体,以A/Puerto Rico/8/34(H1N1)(PR8)的6个内部基因为骨架,利用反向遗传操作技术,救获了一株重组H9N2亚型禽流感病毒疫苗候选株Re-9。该疫苗候选株既具有很好的抗原性又具有较高的鸡胚增殖特性,从而为有效防控H9N2亚型禽流感的流行提供了技术储备。H9N2 subtype avian influenza viruses （AIVs） are widely distributed in avian species. It not only causes poultry disease but the events about this subtype AIV infecting human have been reported occasionally, which threatens the healthy development of poultry industry and public health. To prepare the candidate vaccine against H9N2 subtype AIV, a reassortant virus named RE-9 containing the HA and NA genes from A/chicken/Hunan/S933/2008 （H9N2） and six internal genes from A/Puerto Rico/8/34 （H1N1） was constructed by reverse genetics technology. The result shows that the candidate vaccine has properties of specific subtype antigenicity and adaption in chicken embryos and it provides technologies to prevent H9N2 subtype avian influenza virus epidemic effectively.

关 键 词：H9N2 禽流感病毒 反向遗传 疫苗候选株 

分 类 号：S852.65[农业科学—基础兽医学]