丙泊酚对荷瘤大鼠肿瘤肺转移及MTA1和Wnt1表达的影响  

Effects of propofol on pulmonary metastasis of intravenous injected tumor cells and expressions of MTA1 and Wnt1 in rats

作　　者：张雅静[1] 林春水[1] 汪威[1] 陈莺[1] 

机构地区：[1]南方医科大学南方医院麻醉科,广东广州510515

出　　处：《南方医科大学学报》2014年第7期1011-1015,共5页Journal of Southern Medical University

基　　金：广东省科技计划项目(2012A030400014);广州市科技计划项目(12C22121552)

摘　　要：目的探讨不同剂量丙泊酚对大鼠MADB106细胞肺转移及肿瘤组织中MTA1和Wnt1表达的影响。方法 Fischer344雄性大鼠40只,随机分为:生理盐水组(S组);脂肪乳剂组(F组);丙泊酚30 mg/kg组(P30组)和50 mg/kg组(P50组),每组10只。1%戊巴比妥钠50 mg/kg腹腔注射经股静脉分别泵入等容量上述药物,1 h后均静注0.5 ml MADB106肿瘤细胞(2×105个)。3周后处死,计数肺转移瘤数目;免疫组化检测肺肿瘤组织中MTA1和Wnt1的表达,IPP软件定量分析。结果 F组和S组肺转移瘤数目及肿瘤组织中MTA1和Wnt1的表达无显著差异(P>0.05)。与S组相比,P30和P50组的肺转移瘤数目及肿瘤组织中MTA1和Wnt1的表达均显著减少(P<0.01),肺转移瘤数目及MTA1和Wnt1的表达与丙泊酚的剂量负相关,Pearson相关系数分别为-0.879、-0.980和-0.916(P<0.01)。MTA1和Wnt1的表达呈正相关,Pearson相关系数为0.902(P<0.01)。结论丙泊酚呈剂量依赖性抑制MADB106肿瘤细胞肺转移,并下调转移瘤中MTA1和Wnt1的表达。Objective To investigate the effects of different doses of propofol on pulmonary metastasis of intravenous injected tumor cells and expression of MTA1 and Wnt1 in the metastatic tumor in rats. Methods Forty male Fischer344 rats were randomly divided into 4 equal groups for intravenous administration of normal saline, intralipid, or propofol at the dose of 30 or 50 mg/kg pumped via the femoral vein. One hour after the infusion, MADB106 tumor cells （2×10^5） were injected intravenously in the rats. Pulmonary metastasis of the tumor cells was observed and the expression of MTA1 and Wnt1 in the metastatic tumor detected by immunohistochemistry 3 weeks later. Results The rats receiving saline and intralipid treatments showed a comparable number of pulmonary metastasis and similar expression levels of MTA1 and Wnt1 in the metastatic tumor （P〉0.05）; the tumor number and MTA1 and Wnt1 were significantly lower in the two propofol groups （P〈0.01）. The doses of propofol was inversely correlated with the number of pulmonary metastasis （r=-0.879） and expressions of MTA1 （r=-0.980） and Wnt1 （r=-0.916） （P〈0.01）, and MTA1 and Wnt1 expression levels in the metastatic tumors were closed correlated （r=0.902, P〈0.01）. Conclusion Propofol can dose-dependently suppress pulmonary metastasis of intravenously injected tumor cells and down-regulate MTA1 and Wnt1 expressions in the metastatic tumor tissue.

关 键 词：丙泊酚 肿瘤转移 MTA1 WNT1 

分 类 号：R734.2[医药卫生—肿瘤]