穗花杉双黄酮通过影响caspase-3和β-catenin表达诱导结肠癌细胞SW480凋亡  

Amentoflavone induces apoptosis in SW480 human colorectal cancer cells via regulating β-catenin and caspase-3 expressions

作　　者：杨雨[1] 徐文娟[1] 彭康[1] 孙学刚[1] 

机构地区：[1]南方医科大学中医药学院分子生物学实验室,广东广州510515

出　　处：《南方医科大学学报》2014年第7期1035-1038,共4页Journal of Southern Medical University

基　　金：国家自然科学基金面上项目(81273621);高等学校博士学科点专项科研基金(20134433110007)~~

摘　　要：目的观察穗花杉双黄酮对人结肠癌细胞SW480增殖及凋亡相关因子caspase-3、β-catenin的影响,初步探讨其诱导结肠癌细胞凋亡的作用机制。方法 MTT细胞增殖试验检测穗花杉双黄酮对SW480细胞活力的影响,流式细胞术检测其对SW480细胞凋亡的诱导作用,Western blot法检测穗花杉双黄酮作用细胞后caspase-3、β-catenin蛋白的表达。结果穗花杉双黄酮对SW480细胞生长有抑制作用,具有剂量依赖性。150μmol/L穗花杉双黄酮明显促进其凋亡,凋亡率显著高于对照组(P<0.01)。Western bolt结果表明,穗花杉双黄酮干预可以使caspase-3表达明显增强,β-catenin表达明显减弱。结论穗花杉双黄酮可诱导人结肠癌细胞SW480凋亡,并影响凋亡相关因子caspase-3、β-catenin的表达,从而达到抗肿瘤作用。Objective To investigate the role of β-catenin and caspase-3 in amentoflavone-induced apoptosis of human colorectal cancer SW480 cells. Methods MTT assay was used to detect the viability of SW480 cells exposed to amentoflavone, and flow cytometry was employed to assess the cell apoptosis. Western blotting was performed to determine the protein expressions ofβ-catenin and caspase-3 in the exposed cells. Results Amentoflavone dose-dependently inhibited the viability of SW480 cells, and a high concentration of amentoflavone （150μmol/L） obviously induced apoptosis of the cells. Amentoflavone exposure caused significantly increased expression of caspase-3 and suppressedβ-catenin expression in the cells. Conclusion Amentoflavone-induced apoptosis in SW480 human colorectal cancer cells is associated with altered expressions ofβ-catenin and caspase-3.

关 键 词：穗花杉双黄酮 SW480 CASPASE-3 Β-CATENIN 

分 类 号：R735.35[医药卫生—肿瘤]