偏侧咀嚼大鼠咬肌中过氧化物酶体增殖物受体γ共激活因子1α及细胞凋亡的变化  

Study of peroxisome proliferator-activated receptor-γcoactivator-1α expression and cytoapoptosis in masseter muscles of unilateral chewing rat

作　　者：杨盈盈[1] 丁婷婷[1] 吴庆亭 孔静静[1] 祁冬[1] 汲平[1] 

机构地区：[1]山东大学口腔医学院修复科·山东省口腔生物医学重点实验室,济南250012

出　　处：《中华口腔医学杂志》2014年第7期408-411,共4页Chinese Journal of Stomatology

基　　金：基金项目：高等学校博士学科点专项科研基金（20120131110074）

摘　　要：目的 探讨偏侧咀嚼大鼠咬肌中过氧化物酶体增殖物受体γ共激活因子1α（peroxisome proliferator-activated receptor-γcoactivator-1α,PGC-1α）表达及肌细胞凋亡与咀嚼肌变化的机制.方法 将36只Wistar雌性大鼠用随机数字表法分成2、4、6、8周4个时间点,每一时间点9只,其中6只作为建模组,拔除上颌左侧磨牙建立偏侧咀嚼动物模型,3只作为对照组.应用原子吸收分光光度法检测各组Ca2＋含量;用实时荧光定量PCR法检测咬肌组织中PGC-1α mRNA的相对表达量;用Hoechst染色检测细胞凋亡.结果 建模早期建模组大鼠拔牙侧Ca2＋含量升高且高于对照组,4周时达峰值[（43.62±2.36） μg/g];4周时建模组PGC-1α mRNA的相对表达量达峰值[拔牙侧（1.57±0.10）、非拔牙侧（1.92±0.06）],6、8周时表达量逐渐下降[拔牙侧（1.06±0.08）、（1.08±0.07）,非拔牙侧（1.09±0.10）、（1.11±0.08）];非拔牙侧细胞凋亡率随时间延长而上升,并在6周时达峰值[（38.56±1.64）％].结论 PGC-1α和肌细胞凋亡参与偏侧咀嚼后咬肌的组织改建,并在偏侧咀嚼不同时期发挥作用.Objective To investigate the changes of peroxisome proliferator-activated receptor-γ coactivator-1 α （PGC-1 α） mRNA and cytoapoptosis in the rats＇ masseter muscle which had been influenced by unilateral chewing,and to explore the theoretical foundation of changes in masticatory muscles induced by unilateral chewing.Methods The animal models were established by extracting the Wistar rats＇ left maxillary molars.Thirty-six female Wistar rats were randomly divided into four groups of 2,4,6 and 8 weeks,nine each.In each group there were six rats with molar extracted and three as control.The Ca2＋ level was detected by atomic spectrophotometric method.The relative expression of PGC-1α mRNA was detected by real-time fluorescent quantitative PCR.The apoptosis index was detected by Hoechst staining.Results The Ca2＋ level in the muscle on the extraction side were significantly higher than that in the controls in the beginning stage of unilateral chewing,and reached the peak at the 4th week [（43.62 ± 2.36） μg/g].The relative expressions of PGC-1α increased from the beginning and reached the maximum level at the 4th week [extraction side：（1.57±0.10）; non-extraction side：（1.92±0.06）],while the relative expressions of PGC-1α in 6 and 8 weeks decreased gradually [extraction side：（1.06 ±0.08）,（1.08 ±0.07）; non-extraction side：（1.09±0.10）,（1.11 ± 0.08）].The changes of apoptosis index on non-extraction side increased continually and peaked at the 6th week [（38.56± 1.64）%].Conclusions PGC-1α and cytoapoptosis played important roles in different stages of tissue remodeling induced by unilateral chewing.

关 键 词：咬肌 过氧化物酶体增殖物激活受体 细胞凋亡 

分 类 号：R781[医药卫生—口腔医学]