转化生长因子β对A431细胞上皮向间质转换相关分子表达的影响  

Effects of transforming growth factor-β on the expressions of epithelial-to-mesenchymal transition-related molecules in A431 cells

作　　者：郭圆圆[1] 彭斌 向桂琼[1] 耿松梅[1] 

机构地区：[1]西安交通大学第二附属医院皮肤科,710004

出　　处：《中华皮肤科杂志》2014年第7期490-493,共4页Chinese Journal of Dermatology

基　　金：国家自然科学基金面上项目（81372912）;教育部新世纪人才支持计划（NCET-10-0673）;西安交通大学第二附属医院基金[YJ（ZD）201219]

摘　　要：目的研究转化生长因子β（TGF-β）对人表皮鳞状细胞癌A431细胞上皮向问质转换（EMT）相关分子表达的影响。方法用含7．5μg／LTGF—β处理A431细胞72h后观察细胞形态，采用实时定量PCR和Western印迹分别在mRNA水平和蛋白水平检测EMT相关基因E钙黏着蛋白、N钙黏着蛋白、波形蛋白、β联蛋白表达水平的变化。未经TGF—β处理的A431细胞为对照组。结果TGF—β处理后，A431细胞独立分散，呈梭形。实时定量PCR结果显示，E钙黏着蛋白表达量为未经TGF—β处理组的31．7％，N钙黏着蛋白、波形蛋白、β联蛋白表达量分别为未经处理组的2．475、11．340、2．615倍；Western印迹结果，N钙黏着蛋白、波形蛋白、β联蛋白表达量上升，E钙黏着蛋白表达量下降。结论TGF—β可诱导A431细胞产生EMT现象，TGF—β表达量上升，可能参与表皮鳞状细胞癌发生侵袭、转移。Objective To evaluate the effect of transforming growth factor-β（TGF-β） on the expressions of epithelial-to-mesenchymal transition （EMT）-related molecules in human A431 epidermal squamous cell carcinoma cells. Methods Cultured A431 cells were classified into two groups： TGF-β group treated with TGF-β of 7.5 μg/L for 72 hours, and control group remaining untreated. Subsequently, cell morphology was observed under an inverted microscope, and real time-PCR and Western blot were performed to quantify the mRNA and protein expressions of EMT-related molecules including E-cadherin, N-cadherin, vimentin and β-catenin respectively. Results After TGF- β treatment, the cells became dispersed and spindle in shape. The mRNA expression levels of E-cadherin, N- cadherin, vimentin, and β-catenin in TGF-β group were 0.317, 2.475, 11.340 and 2.615 folds those in the control group respectively. Western blot showed a marked increase in the expression of N-cadherin, vimentin, and β-catenin proteins but a notable decrease in that of E-cadherin in the TGF-β group compared with the control group. Conclusions TGF-β can induce EMT in A431 cells, and increased expression of TGF-β may contribute to the invasion and metastasis of SCC.

关 键 词：细胞系 肿瘤 转化生长因子Β 上皮向间质转化 

分 类 号：R739.5[医药卫生—肿瘤]