多西环素对乳腺癌小鼠移植瘤生长及转移的影响  

Effects of Doxycycline on Tumor Growth and Metastasis in Breast Cancer Xenografts in Mice

作　　者：赵蕴珍[1] 李佳怡[1] 

机构地区：[1]天津医学高等专科学校,300222

出　　处：《天津医药》2014年第7期667-669,共3页Tianjin Medical Journal

基　　金：天津市高等学校科技发展基金计划项目(20060124)

摘　　要：目的通过检测多西环素对小鼠乳腺癌肺转移模型(BCML-TAⅡ99)中基质金属蛋白酶-9(MMP-9)及金属蛋白酶组织抑制因子1(TIMP-1)蛋白表达水平,揭示其对该肿瘤生长及转移的影响。方法建立BCML-TAⅡ99模型,应用免疫组织化学SP法检测MMP-9和TIMP-1蛋白在多西环素给药组与对照组肿瘤组织中的表达情况,并进行比较。结果与对照组相比,给药组肿瘤转移率明显低于对照组;MMP-9蛋白在给药组表达下调,而TIMP-1蛋白表达上调,差异具有统计学意义(P<0.05),且MMP-9与TIMP-1表达呈负相关(P<0.05)。结论多西环素可通过调节MMP-9及TIMP-1蛋白表达水平,对BCML-TAⅡ99模型中肿瘤生长及转移发挥抑制作用。Objective To investigate the effects of doxycycline on the expressions of matrix metalloproteinase-9 （MMP-9） and tissue inhibitor of metaloproteinase-1 （TIMP-1） in breast cancer lung metastasis model mice （BCML-TAⅡ99）, and the mechanism thereof. Methods The BCML-TAⅡ99 mouse model was established. The immunohistochemical staining was performed to detect the expressions of MMP-9 and TIMP-1 in BCML-TAⅡ99 model mice and controls. Results The metastasis rate was significantly lower in treatment group than that of control group. The expression of MMP-9 was significantly reduced, but the expression of TIMP-1 was significantly increased, in treatment group compared with those of control group （P〈0.05）. Conclusion Doxycycline can inhibit tumor growth and metastasis in breast cancer with 1ung metastasis model mice by regulating MMP-9 and TIMP-1 expressions.

关 键 词：多西环素 乳腺肿瘤 肿瘤转移 肺 基质金属蛋白酶9 金属蛋白酶类组织抑制剂 小鼠 近交系 乳腺癌 

分 类 号：R737.9[医药卫生—肿瘤]