神经型钙黏蛋白在肝门部胆管癌中的表达及意义  

Expression and Significance of Neural Cadherin in Perihilar Cholangiocarcinoma

作　　者：杨景红[1] 董祖海[1] 肖福斌[1] 候巧燕[1] 

机构地区：[1]桂林医学院附属医院肝胆外科,541004

出　　处：《天津医药》2014年第7期680-682,共3页Tianjin Medical Journal

基　　金：广西壮族自治区医疗卫生重点科研课题(2011010)

摘　　要：目的研究神经型钙黏蛋白(N-cadherin)在肝门部胆管癌组织中的表达及临床意义。方法免疫组化检测26例肝门部胆管癌与癌旁及5例正常胆管组织中N-cadherin表达差异。Western blot对比N-cadherin在肝门部胆管癌组织中与癌旁中差异。结果免疫组化示26例肝门部胆管癌癌组织中有14例N-cadherin表达阳性,癌旁组织中6例阳性(P<0.05)。正常胆管阳性表达0例。N-cadherin在低分化癌组阳性表达率高于中、高分化癌组。Western blot示N-cadherin在肝门部胆管癌组织中的表达高于癌旁组织(0.88±0.12 vs 0.43±0.09,P<0.05)。结论 N-cadherin可能参与肝门部胆管癌的发生发展。Objective To explore the expression and the clinical significance of neural cadherin （N-cadherin） in perihilar cholangiocarcinoma. Methods The expression of N-cadherin was detected by immunohistochemical method in 26 patients with perihilar cholangiocarcinoma and 5 samples of normal bile duct tissues. The expression of N-cadherin was also compared by Western blotting assay between perihilar cholangiocarcinoma and paraneoplastic tissues. Results The im-munohistochemical results showed that positive expression of N-cadherin was observed in 14 of 26 patients with perihilar cholangiocarcinoma and in 6 of 26 paraneoplastic samples （53.84%vs 23.08%, P〈0.05）. There was 0 N-cadherin expres-sion in normal bile duct tissues. The positive rate of N-cadherin expression was higher in low differentiated group than that in middle and well differentiated groups. The result of Western blotting assay showed that N-cadherin expression was signifi-cantly higher in perihilar cholangiocarcinoma than that in the paraneoplastic tissues （0.88 ± 0.12 vs 0.43 ± 0.09, P 〈 0.05）. Conclusion N-cadherin may be involved in the occurrence and development of perihilar cholangiocarcinoma.

关 键 词：钙黏着糖蛋白类 胆管肿瘤 免疫组织化学 印迹法 蛋白质 神经型钙黏蛋白 肝门部胆管癌 

分 类 号：R735.8[医药卫生—肿瘤]