外源肌肉生成抑制素蛋白免疫对雄兔促性腺激素的影响  

Effects of Exogenous MSTN Protein on the Gonadotrophins in Male Rabbits

作　　者：陈蓉[1] 曲月秀[1] 石照应[1] 田兴贵[1] 

机构地区：[1]贵州大学动物科学学院,贵州贵阳550025

出　　处：《贵州农业科学》2013年第11期139-141,共3页Guizhou Agricultural Sciences

基　　金：贵州省科技厅农业攻关项目"优质高产小香羊选育和养殖配套技术示范"[黔科合NY字(2008)3046]

摘　　要：为探究肌肉生成抑制素(MSTN)蛋白主动免疫对雄兔促性腺激素合成与分泌的作用,选择15只2月龄雄兔随机分为3组,分别用生理盐水(对照组)、0.26mL MSTN蛋白(试验Ⅰ组)、0.52mL MSTN蛋白(试验Ⅱ组)进行免疫试验。结果表明:MSTN蛋白免疫后,在60d时试验Ⅱ组血清中FSH[(12.16±2.30)IU/L]、LH[(46.77±3.44)ng/L]含量显著(P<0.05)高于对照组,试验Ⅱ组血清中T含量[(477.42±91.93)ng/L]极显著(P<0.01)高于对照组;试验Ⅰ组血清中FSH、T、LH与对照组差异不显著(P>0.05);各组血清中E2均无显著差异(P>0.05),但在45d、60d时试验Ⅱ组中E2略高于对照组。MSTN蛋白在雄性动物生殖中具有促进作用。In order to explore the synthesis and secretion of gonadotrophins and estrogen in male rabbits,which were immunized by exogenous MSTN protein,Fifteen 2-month-old male rabbits were randomly assigned into three groups.They were immunized with normal saline （control group）,0.26 mL MSTN protein （Group Ⅰ） and 0.52 mL MSTN protein （Group Ⅱ）,respectively.Results：After immunized with MSTN protein,the FSH and LH had the significant differences between Group Ⅱ and the control group on the sixtieth days （P＜0.05） ; The T had highly significant differences between Group Ⅱ and the control group on the sixtieth days（P＜0.01）; The FSH,T and LH had no significant difference between Group Ⅰ and the control group （P＞ 0.05） ; Serum estradiol levels had no significant difference among all groups（P＞0.05）.The results suggested that MSTN had a role in male animal reproduction.

关 键 词：雄兔 外源MSTN蛋白 卵泡刺激素 黄体生成素 雌二醇激素 睾酮 

分 类 号：S811.2[农业科学—畜牧学]