荞麦子叶节再生体系的建立  

Establishing of Regeneration System in Buckwheat Cotyledon Node

作　　者：黄凤兰[1] 崔荣[1] 曹聪[1] 陈玲玲[1] 孙美丽[1] 雷雪[1] 王婕[1] 邹千稳[1] 

机构地区：[1]内蒙古民族大学生命科学学院,内蒙古通辽028000

出　　处：《内蒙古农业科技》2014年第3期7-9,共3页Inner Mongolia Agricultural Science and Technology

摘　　要：实验以荞麦子叶节为研究对象,摸索其再生所需的最适条件,建立荞麦的再生体系,为荞麦的转基因研究奠定基础。实验结果表明:种子萌发条件为去掉外种皮的种子用0.1%升汞灭菌1min,30g/L蔗糖+7.5g/L琼脂粉的固体培养基培养。子叶节诱导生芽最适培养基为MS+2.5mg/L6-BA+0.05mg/LNAA+30g/L蔗糖+7.5g/L琼脂粉,芽诱导生根的培养基为1/4MS+6-BA(2.0mg/L)+NAA(0.05mg/L)+30g/L蔗糖+7.5g/L琼脂粉。Using buckwheat cotyledon node as the research object, its optimum conditions of regeneration was aimed to grope, to establish buckwheat regeneration system and lay the foundation for the research of transgenic buckwheat. The results showed that the best disinfection conditions was 0.1% HgCl2 for 1.Omin and 30g/L suerose＋7.5g/L agar medium on peeled buckwheat seeds, the best culture medium for cotyledon node induce buds was MS＋2.5mg/L6-BA＋ 0.05mg/LNAA＋30g/L sucrose＋7.5g/L agar and the best culture medium for buds to induce roots was 1/4MS＋2.0mg/L6-BA＋ 0.05mg/LNAA＋30g/L sucrose＋7.5g/L agar.

关 键 词：荞麦 子叶节 再生体系 

分 类 号：S517[农业科学—作物学]