厌氧氨氧化耦合脱氮系统中反硝化细菌研究  

Denitrifying bacteria of anaerobic ammonium-oxidizing denitrifying system

作　　者：王春香[1] 刘常敬[1] 郑林雪[1] 李军[1] 

机构地区：[1]北京工业大学建筑工程学院,北京100124

出　　处：《中国环境科学》2014年第7期1878-1883,共6页China Environmental Science

基　　金：国家自然科学基金(51078008);北京市自然科学基金(8122005);北京市教委重点项目(KZ201110005008)

摘　　要：采用聚合酶链式反应、分子克隆等分子生物学方法,通过构建nirS克隆文库研究了厌氧氨氧化耦合异养反硝化脱氮系统中的反硝化微生物.进行同源性分析和系统发育树构建,结果表明,从nirS克隆文库中随机挑选的100个克隆子中有51个阳性克隆子,共分为12个操作单元.经比对发现这些微生物主要为变形菌门细菌和未知菌类,其中β-proteobacteria占据绝对优势且有较多种类,少部分属于γ-proteobacteria.Denitrifying microorganisms in the anaerobic ammonium oxidation coupling heterotrophic denitrification system were investigated. The methods used were PCR and molecular cloning. By building nirS clone libraries, homology analysis and constructing system Phylogenetic tree, 51clones were assigned to 12 OTUs. By BLAST alignment found these microbes were mainly proteobacteria and uncultured environment samples.Proteobacteria were mainly β-Proteobacteria andγ-Proteobacteria.

关 键 词：厌氧氨氧化 反硝化菌 聚合酶链式反应(PCR) nirS克隆文库 

分 类 号：X172[环境科学与工程—环境科学]