糯玉米种质SSR标记遗传及品质性状分析  

Analysis of Genetic and Quality Traits of Waxy Corn Germplasms

作　　者：孟强[1] 戴冬青[1] 杨利[2] 李娟[1] 齐望[1] 赵仁贵[1] 

机构地区：[1]吉林农业大学农学院,长春130118 [2]吉林农业大学生命科学学院,长春130118

出　　处：《吉林农业大学学报》2014年第3期258-264,共7页Journal of Jilin Agricultural University

基　　金：国家星火计划项目(2011GA660005);吉林省财政厅科研育种项目(吉财2012002);长春市科技支撑计划项目(长科技合2011215号)

摘　　要：利用SSR标记研究50份糯玉米自交系的遗传变异,从40对核心引物中筛选出扩增条带清晰、稳定性和多态性好的18对引物,在50份糯玉米自交系中检测出87个等位基因变异,每对引物检测等位基因3～7个,平均4.83个;每对SSR引物的多态信息量（PIC）为0.211～0.759,平均0.584。利用UPGMA聚类分析方法把50份供试糯玉米自交系分为4个类群,其划分结果与系谱分析基本一致。分别测定了50份糯玉米自交系子粒中蛋白质、淀粉、水分、脂肪和游离氨基酸的含量,结果表明：糯玉米子粒营养成分含量各不相同,游离氨基酸变异系数最大,淀粉变异系数最小,具有丰富的遗传多样性。基于50份自交系的品质性状数据,利用SPSS分析软件中平方欧式距离聚类分析方法进行聚类,结果划分为2个大类,4个亚类,类群之间具有较大差异。The SSR markers were used to study the genetic variation of 50 waxy maize inbred lines,and 18 pairs of primers were selected from 40 pairs of primers.A total of 87 polymorphic bands,variation of 3 ～ 7 alleles for each primer,and an average of 4.83 alleles per primer were detected;polymorphic information content of each pair of SSR primers ranged from 0.211 to 0.759 averaged0.584.Using clustering analysis in UPGMA,the waxy corn inbred lines were classified into 4groups,which was basically the same as germplasmic genealogy.The contents of protein,starch,moisture,fat and free amino acids in the grain of waxy maize inbred lines were determined.The results showed that grain nutrient content of each waxy maize was not identical,free amino acid variation coefficient was the maximum and that of the starch was the minimum,indicating that the inbred lines were of rich genetic diversity.Based on the data of the quality traits in the 50 inbred lines,adopting the squared Euclidean distance cluster analysis,the 50 waxy maize inbred lines were divided into 2 groups,4 subgroups,and the there were significant differences among the groups.

关 键 词：糯玉米 SSR 遗传多样性 子粒品质 

分 类 号：S513.032[农业科学—作物学]