CaDREB3启动子分离及其缺失体转基因烟草的获得  

Isolation of CaDREB3 Promoter and Acquisition of Transgenic Tobacco Plants for Its 5' Deletion Mutation Analysis

作　　者：蔡金森[1,2] 王博[1,2] 杨晟[1,2] 石兰平[1,2] 文嘉瑜 史玮[1,3] 刘志钦[1,2] 贺俐[4] 吴杨[4] 何水林[1,2] 

机构地区：[1]福建农林大学作物遗传育种与综合利用教育部重点实验室,福建福州350002 [2]福建农林大学生命科学学院,福建福州350002 [3]福建农林大学作物科学学院,福建福州350002 [4]井冈山大学生命科学学院,江西吉安343009

出　　处：《热带作物学报》2014年第7期1368-1373,共6页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金项目(No.31060263;31260482)

摘　　要：CaDREB3是辣椒DREB家族中1个成员,为明确CaDREB3受逆境胁迫诱导表达的分子机制,从辣椒基因组DNA中分离获得了CaDREB3上游的启动子,利用生物信息学软件进行顺式作用元件的分析,结果表明该启动子的TATA框为起始密码子ATG上游-89 bp至-86 bp的TATA,同时含有多种与逆境胁迫相关的顺式作用元件,如ABRE、HSE、MYB和TCA等,并使用Gateway技术构建了启动子6个缺失体的GUS融合载体,获得了6个缺失体的烟草转基因植株,这些结果为将来分析该启动子应答逆境胁迫的调控区域奠定了一定的基础。CaDREB3 is a member of the DREB family in pepper.In order to further clarify the molecular mechanism of the stress-induced expression of CaDREB3,we isolated the upstream promoter of CaDREB3 from pepper genomic DNA.Bioinformatics analysis illustrated that the TATA box located from-89 bp to-86 bp,upstream from start codon （ATG）,and a variety of stress-associated cis-acting elements were included,such as ABRE,HSE,MYB and TCA,etc.Five deletions fused to GUS--reported gene of CaDREB3 promoter were constructed via Gateway technology and transgenic plants of the six deletions were generated.Taken together,the results above lay the foundation to determine the regulatory region of the promoter response to stress.

关 键 词：DREB转录因子 GENOME WALKING 启动子 

分 类 号：Q78[生物学—分子生物学]