九节龙皂苷Ⅰ对口腔黏液表皮样癌细胞系M3SP2增殖、迁移和侵袭作用的影响  

Effect of ArdipusillosideⅠon proliferation,migration and invasion of human mucoepidermoid carcinoma cell line M3SP2

作　　者：姜刚勇[1] 

机构地区：[1]济宁市第一人民医院口腔科,山东济宁272100

出　　处：《海南医学院学报》2014年第9期1175-1177,1180,共4页Journal of Hainan Medical University

基　　金：济宁市科技局资助项目(济卫中医字(2012)23号LC2012010)~~

摘　　要：目的:研究九节龙皂苷I对口腔黏液表皮样癌细胞系M3SP2的增殖、迁移和侵袭作用的影响。方法:培养口腔黏液表皮样癌细胞系M3SP2,分为对照组(不含药物的DMEM处理)、九节龙皂苷组(15μg/mL九节龙皂苷I)处理,通过MMT方法检测细胞的活力、划痕试验检测细胞的迁移和侵袭,Western-blot检测Bcl-2、Bax和基质金属蛋白酶2、9(MMP-2、MMP-9)的蛋白含量。结果:(1)增殖、迁移和侵袭能力:九节龙皂苷组的MTT值、(A0-A24)/A0均低于对照组(P<0.05);(2)分子机制:九节龙皂苷组的Bcl-2、MMP-2、MMP-9含量低于对照组,Bax含量高于对照组(P<0.05)。结论:九节龙皂苷Ⅰ可以抑制黏液表皮样癌细胞的增殖、迁移和侵袭,这一作用可能是通过上调Bax、下调Bcl-2、MMP2、MMP9来实现的。Objective： To study the effect of Ardipusilloside I on proliferation, migration and invasion of human mucoe- pidermoid carcinoma cell line M3SP2. Methods： Human mucoepidermoid carcinoma cell line M3SP2 were cultured and divided into control group （treated with DMEM） and ardipusilloside group （treated with 15μg/mL ardipusilloside Ⅰ ）. Then vitality was detected by MTT, migration was detected by wound-healing assay and protein level of MMP-2, Caspase-2 was detected by Western blot. Results： MTT value and （A0--A240/A0） of observation group were lower than those control group. Bcl-2, MMP 2, MMP-9 contents of observation group were lower than those of control group, Bax content was higher than that of control group. Conclusion： Ardipusilloside I can inhibit proliferation, migration and invasion of human mucoepidermoid carci noma cell, uDre-ulate Bax, and downreaulate Bcl 2, MMP2, MMP9, which maybe its possible molecular mechanism.

关 键 词：人黏液表皮样癌 九节龙皂苷Ⅰ 增殖 迁移 侵袭 

分 类 号：R739.8[医药卫生—肿瘤]