玉屏风药液对环磷酰胺抑制的C57BL/6小鼠免疫功能的影响  

Effect of Yupingfeng solution on the immune function of C57BL /6 mice inhibited by Cyclophosphamide

作　　者：陈健明[1,2] 陈殿慧[1] 余秀雪[1] 李璐[1] 吴凡[1] 梁辉 黄俊[1] 

机构地区：[1]广州医科大学基础医学院,广东广州510182 [2]广东省广州市番禺区中心医院,广东广州511400 [3]广东省深圳市龙华新区人民医院,广东深圳518109

出　　处：《现代中西医结合杂志》2014年第23期2509-2511,2515,共4页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：广东省医学科研基金资助项目(A2012603);广州市高校科研计划项目(2012C117)

摘　　要：目的观察玉屏风(YPF)药液对环磷酰胺(CY)抑制C57BL/6小鼠免疫功能的影响。方法腹部皮下注射环磷酰胺制作免疫抑制小鼠模型,分别使用玉屏风药液50 mg/d和100 mg/d给C57BL/6小鼠灌胃,连续7 d。7 d以后,眼眶取血,血常规检测血细胞含量;制备脾淋巴细胞悬液,计数;流式细胞仪检测淋巴细胞亚群的含量;使用R848刺激细胞72 h,MTT法检测细胞的增殖情况。结果玉屏风药液可以抑制环磷酰胺诱导的小鼠血液中白细胞减少,部分缓解环磷酰胺所致的脾淋巴细胞减少,增加脾细胞中B细胞、NK细胞的含量,部分提高环磷酰胺免疫抑制小鼠脾淋巴细胞的增殖能力。结论玉屏风药液能够部分缓解环磷酰胺对C57BL/6小鼠免疫功能的抑制作用。Objective It is to observe the effect of Yupingfeng （YPF） solution on the immune function of C57BL/6 mice treated with Cyclophosphamide .Methods Cyclophosphamide was given to C57BL/6 mice by abdominal subcutaneous injection to establish inhibit immune function models which were given YPF solution at dose of 50mg/d and 100 mg/d respectively by intragastric administration for 7 days.Blood tests were used to count the percentage of blood cells in different groups of mice . Microscope was used to count spleen cells in different groups of mice .The cell surface stain method was used to observe the percentage of lymphocyte subsets .MTT assay was used to observe cell proliferation after simulation for 72 h with R848 .Re-sults YPF can inhibit Cyclophosphamide-induced neutropenia , partly inhibit the reduction of mouse spleen lymphocytes , in-crease the contents of B cell , NK cell, partly promote lymphocytes proliferation in cyclophosphamide-treated mice.Conclu-sion YPF solution can partially relieve the inhibition effect of Cyclophosphamide in immune function of C 57BL/6 mouse.

关 键 词：玉屏风药液 环磷酰胺 脾脏 淋巴细胞 增殖 

分 类 号：R-332[医药卫生]