豨莶草叶浸提液对羟基自由基的清除作用  

Scavenging effect of Siegesbeckia leaves extracts on hydroxyl radical

作　　者：张成[1] 蔡曙洁 陈炜玥 邓娜梅 韩宇浩 陈超[1] 

机构地区：[1]北京师范大学珠海分校工程技术学院,广东珠海519087 [2]北京师范大学生命科学学院,北京100875

出　　处：《南方农业学报》2014年第6期1060-1064,共5页Journal of Southern Agriculture

基　　金：北京师范大学珠海分校青年教师科研项目(2013);北京师范大学珠海分校大学生创新创业训练项目(2014);北京师范大学珠海分校生物技术品牌专业建设项目(201262)

摘　　要：【目的】研究豨莶草叶浸提液对羟基自由基(·OH)的清除作用,为豨莶草资源的药用开发提供参考依据。【方法】采用水浴回流法制备豨莶草叶浸提液,通过单因素试验和正交试验,确定豨莶草叶浸提液清除·OH效果最佳的浸提工艺条件。【结果】影响豨莶草叶浸提液清除·OH效果的因素顺序为:料液比>乙醇体积分数>水浴温度>浸提时间,其最佳浸提工艺条件:以80%乙醇为浸提剂,在料液比1∶10.0、60℃水浴回流条件下浸提2.0 h,·OH清除率为91.7%;豨莶草叶浸提液体积为1.00 mL时,·OH清除率达92.6%。【结论】豨莶草叶浸提液具有一定的抗氧化性,可作为天然抗氧化剂资源进一步研究。[Objective ]The scavenging effect of Siegesbeckia leaves extracts on hydroxyl free radical （. OH） was studied to provide references for its further development in medicinal aspect. [Method]Siegesbeckia leaves extracts were obtained by refluxing in the water-bath. Using single factor and orthogonal test methods, the optimal extraction conditions of scavenging ratio of Siegesbeckia leaves extracts on hydroxyl free radical were determined. [Result]The factors affecting the scavenging effect of the Siegesbeckia leaves extracts on hydroxyl free radical were found in the following order： material liquid ratio 〉 ethanol volume percentage 〉 water-batb temperature 〉 extraction time. Under the optimal extraction conditions of 1：10.0 of material liquid ratio, 80% ethanol, and 2.0 hours at 60℃ refluxing temperature, the maximum capacity of scavenging effect to hydroxyl free radical was 91.7%. When Siegesbeckia leaves extracts were used as 1.0ml, the scavenging ratio to hydroxyl free radical was 92.6%. [Conclusion]Siegesbeckia leaves extracts have antioxidant effects, which shows that Siegesbeckia leaves extracts should be used as natural antioxidant resources for further study.

关 键 词：豨莶草叶浸提液 羟基自由基(·OH) 清除率 抗氧化性 提取工艺 

分 类 号：TQ282[化学工程—有机化工]