FTIR-NIR研究镉胁迫对构树试管幼苗生长的影响与机理  

FTIR-NIR Spectrum Study on Response and Mechanism of Broussonetia papyrifera Sprout Under Cadmium Stresses

作　　者：王成[1,2] 蒋泽平[3] 李文青[1] 邵文静 张婷[2] 季峻峰[2] 

机构地区：[1]南京市环境保护科学研究院,南京210013 [2]表生地球化学教育部重点实验室,南京大学地球科学与工程学院,南京210093 [3]江苏省林业科学研究院,南京210018

出　　处：《农业环境科学学报》2014年第4期673-679,共7页Journal of Agro-Environment Science

基　　金：国家"十一五"科技支撑项目"耐盐经济林木良种选育"(2006BAD09A04-01-06);南京市环保科技项目"典型工农业交错区土壤镉生物有效性影响因素及污染防治措施研究"(201306)

摘　　要：利用傅里叶转换漫反射红外光谱(FTIR)和近红外光谱(NIR)测定了构树幼苗在不同浓度镉胁迫下叶片的红外光谱,揭示了构树叶片中蛋白质、脂类等有机化合物合成对镉胁迫的生理响应。结果表明,构树幼苗在Cd胁迫下,鲜叶红边位置发生"蓝移"现象,红边蓝移程度与叶绿素含量呈正相关关系。近红外光谱的710 nm波长可作为是否受胁迫的红边位置界限。镉胁迫下构树幼苗的FTIR光谱3010 cm-1、1720 cm-1、1636 cm-1、1468 cm-1、892 cm-1处特征吸收峰强度随胁迫强度增加而增加,揭示了构树试管苗叶片通过合成更多的蛋白质、纤维素、羧酸以及部分酯类有机物来响应镉胁迫。FTIR-NIR光谱分析可用于植物对Cd胁迫的生理学研究,并可作为一种快捷方法应用于植物重金属污染监测。Broussonetia papyrifera,a popular tree in China,is tolerant to barren and drought soil conditions.It is also potential pollution-resistant.In this study,Fourrier Transform Infrared Spectrometry（FTIR）and Near Infrared Spectrum（NIR）were applied to study the dynamics of protein and other organic components in test-tube Broussonetia papyrifera sprout under different Cd stresses.Chlorophyll contents in Broussonetia papyrifera sprout leaves were also analyzed.Cadmium concentrations in sprout leaves increased,but chlorophyll contents decreased with increasing Cd stresses.Under Cd stresses,The red edge position in fresh leaves of Broussonetia papyrifera sprout exhibited＂blue-shift＂,and the degree of the＂blue-shift＂showed a significant correlation with chlorophyll concentration.The red edge position of wavelength 710 nm in NIR can be used as an indicator of plant pollution by Cd.The intensities of absorption peaks 3010 cm^-1,1720 cm^-1,1636 cm^-1,1468 cm^-1and 892 cm^-1in FTIR increased with Cd stresses,implying that Broussonetia papyrifera sprout synthesized more protein,polysaccharide,ester and other organic components under Cd stresses.FTIR-NIR is a useful technique to study the mechanisms of plant responses to heavy metal stress and is also a potentially quick method for monitoring plant heavy metal pollution.

关 键 词：红外光谱 构树 镉胁迫 生理作用 叶绿素 化学组分 

分 类 号：X171.5[环境科学与工程—环境科学]