利用固定波长荧光分光光度法研究苯并(a)芘暴露下罗非鱼胆汁代谢物的动态变化  

Dynamics of Benzo(a)Pyrene Metabolites in Tilapia Bile by Fixed Wavelength Fluorescence to:A Lab Experiment

作　　者：宋超[1] 裘丽萍[1] 范立民[1] 孟顺龙[1] 贾旭淑[2] 胡庚东[1] 陈家长[1,2] 

机构地区：[1]中国水产科学研究院淡水渔业研究中心中国水产科学研究院内陆渔业生态环境和资源重点开放实验室,江苏无锡214081 [2]南京农业大学无锡渔业学院,江苏无锡214081

出　　处：《农业环境科学学报》2014年第4期783-787,共5页Journal of Agro-Environment Science

基　　金：国家现代农业产业技术体系项目(CARS-49);中央级公益性科研院所基本科研业务费专项资金(2011JBFA16)

摘　　要：利用固定波长荧光分光光度法(Fixed-wavelength fluorescence,简称FF法)研究了苯并(a)芘暴露下罗非鱼胆汁代谢物的动态变化。在试验中首先优化了FF法测定罗非鱼胆汁代谢物的稀释倍数,将罗非鱼胆汁分别稀释1000、2000、4000、8000倍,在激发/散发波长380 nm/430 nm测定荧光强度。结果显示2000倍左右的稀释较为合理,结合其他研究,试验最终采用1600倍的稀释倍数。在此基础上利用优化后的FF法研究了不同浓度的BaP(0.1、1、10、50μg·L-1)暴露下罗非鱼胆汁代谢物的动态变化。结果显示:0.1μg·L-1浓度组的BaP代谢物随时间变化无明显波动,与对照组无显著差异(P>0.05);其他3个剂量组从2 h起就与对照组差异显著(P<0.05),3个试验组均呈现先上升后下降的趋势。胆汁中BaP代谢物随浓度升高不断升高,呈现明显的剂量-效应关系,表明鱼体内胆汁代谢物可用来反映周围水环境中BaP的污染情况。这为将FF法应用于环境中BaP的生物监测,使胆汁代谢物成为BaP环境监测的有效生物标志物提供了理论依据。Biomarker plays an important role in environmental pollution monitoring.Here dynamics of benzo（a）pyrene（BaP）metabolites in tilapia bile were monitored under artificial exposure using fixed-wavelength fluorescence method（FF）.Dilution rate of tilapia bile for the FF method was first developed by diluting bile at 1∶1000,1∶2000,1∶4000,and 1∶8000 and setting excitation/emission wavelength of spectrofluorophotometer at 380 nm/430 nm.Dilution rate at 2000-fold was found to be reasonable.Combined with other studies,1600-fold dilution was selected in the present experiment to examine the dose and time effects on dynamics of BaP metabolites in bile（0.1 μg·L^-1,1 μg·L^-1,10 μg·L^-1and 50 μg·L^-1）.At 0.1 μg BaP·L^-1,BaP metabolites neither showed obvious fluctuation nor had significantly differences from the control group（P〉0.05）.However,significant differences were observed between BaP metabolites at the other three doses and the control,beginning from 2 h（P〈0.05）.The fluorescence intensity showed＂rise then fall＂trend at concentrations of 1 μg·L^-1,10 μg·L^-1,and 50 μg·L^-1.The metabolites of BaP in bile had a significant dose-response relationship.These results demonstrate that BaP dynamics in tilapia bile are certainly sensitive biomarkers to assess fish exposure to BaP and FF method can be widely used in the environment monitoring.

关 键 词：苯并(A)芘 胆汁代谢物 固定波长荧光分光光度法 生物标志物 

分 类 号：X171.5[环境科学与工程—环境科学]