苯胺降解菌Rhodococcus sp.E2的分离与苯胺氧化酶基因簇的初步鉴定  

Isolation of Aniline-degrading Bacterium Rhodococcus sp. E2 and Characterization of Aniline Dioxygenase Gene Cluster

作　　者：李云翔[1] 陈雪婷[1] 周潮洋 裴涛[1] 刘永闯 洪青[1] 闫新[1] 李顺鹏[1] 

机构地区：[1]南京农业大学生命科学学院,南京210095

出　　处：《农业环境科学学报》2014年第5期882-889,共8页Journal of Agro-Environment Science

基　　金：国家基础科学人才培养基金(J1210056)

摘　　要：从江苏省南通市某农药厂的活性污泥中分离出一株苯胺降解菌株E2,根据表型特征、生理生化特性和16S rDNA序列分析,将其鉴定为红球菌属(Rhodococcus sp.)的细菌。菌株E2降解苯胺的最适温度为30℃、最适pH为7.0,降解苯胺的最高浓度为800 mg·L-1。该菌能降解苯胺、邻甲基苯胺、对甲基苯胺和2,5-二氯苯胺,与已报道的苯胺降解菌的底物谱显著不同。通过扩增高度保守的片段和染色体步移,获得了菌株E2中负责苯胺降解的基因簇,该基因簇在基因组成、排布及同源性方面和已报道的基因簇有较大差异,是研究苯胺降解分子机理的较好材料。Aniline and its derivatives have become environmental pollutants. The existing aniline-degrading bacteria have narrow substrate spectrum toward aniline derivatives and can degrade parts of these compounds. Here we isolated an aniline-degrading bacterium E2 from activated sludge originated from an insecticide factory, and revealed its molecular nature. The strain E2 was identified as Rhodococcus sp. based on morphological, physiological and biochemical properties, and homology analysis of 16S rDNA sequence. The optimum temperature and pH value for the degradation by the strain E2 were 30 ℃ and 7.0, respectively. The highest aniline concentration at which the strain E2 could degrade was 800 mg· L-1 . It could degrade aniline, o-Toluidine, p-Toluidine and 2,5-dichloroaniline. Aniline-degrading gene cluster was cloned from the strain E2 by amplifying the highly conserved fragment and chromosome walking. This cluster was significantly different from the current ones in composition, arrangement and homology of genes. It would be a valuable material for investigating the molecular mechanisms of aniline degradation.

关 键 词：苯胺 RHODOCOCCUS SP E2 基因簇 

分 类 号：S154.3[农业科学—土壤学]