苹果叶片应答斑点落叶病菌胁迫的蛋白质组学分析  

Proteome analysis of pathogen-responsive proteins from apple leaves induced by Alternaria alternata apple pathotype

作　　者：张彩霞 张利义[1,2] 田义 陈莹[1,2] 康国栋 丛佩华[1,2] 王强 杨玲[1,2] 李武兴 

机构地区：[1]农业部园艺作物种质资源利用重点实验室,辽宁兴城125100 [2]中国农业科学院果树研究所,辽宁兴城125100

出　　处：《植物病理学报》2014年第4期438-442,共5页Acta Phytopathologica Sinica

基　　金：农业部现代农业产业技术体系建设专项资金(CARS-28);国家自然科学基金资助项目(30900968;31201602);国家"863"项目(2011AA10020404);国家科技支撑计划项目(2013BAD02B01)

摘　　要：苹果斑点落叶病（Apple Alternaria Blotch）又称褐纹病，是目前我国苹果主产区的主要病害之一。病原菌是植物发病的主要生物胁迫因子。目前，植物寄主应答病原真菌胁迫的蛋白质组学研究在拟南芥、水稻等模式植物中已广泛展开。In order to elucidate key proteins related to disease-resistance in apple leaves, the total proteins were extracted from control and infected apple leaves, and separated by two-dimensional electrophoresis. The differential expressed proteins were then identified by MALDI-TOF-TOF/MS. In total, 25 differential expressed proteins were detected by Image Master Software. After tryptic digestion, MALDI-TOF-TOF/MS analysis and database searching, 20 protein spots were finally identified, including 11 functional proteins related to photosynthesis, energy metabolism, stress and defense responses. The pathogenesis-related proteins involved in defense responses such as APX, GPX and Mal dl were differentially expressed in apple leaves. It indicates that these proteins may play a key role in the resistance to A. alternata apple pathotype.

关 键 词：苹果斑点落叶病 蛋白质组学 胁迫因子 苹果叶片 应答 学分 病菌 植物寄主 

分 类 号：S436.611.1[农业科学—农业昆虫与害虫防治]