肠炎沙门氏菌SDA-琼脂糖凝胶电泳检测技术研究  

Detection of Salmonella enteritidis by Strand Displacement Amplification and Agarose Gel Electrophoresis

作　　者：尼秀媚[1] 魏晓棠[1] 

机构地区：[1]山东出入境检验检疫局,山东青岛266002

出　　处：《安徽农业科学》2014年第22期7443-7444,7466,共3页Journal of Anhui Agricultural Sciences

基　　金：国家质检总局项目(2012IK282);山东出入境检验检疫局项目(SK201206)

摘　　要：[目的]建立肠炎沙门氏菌的SDA快速检测方法。[方法]根据肠炎沙门氏菌invA基因片段设计链置换扩增(SDA)引物,建立肠炎沙门氏菌的SDA快速检测方法,并对设计的引物的灵敏度和特异性进行研究。[结果]成功建立了肠炎沙门氏菌的SDA快速检测方法,SDA反应成功扩增了目标序列。建立的SDA检测技术对肠炎沙门氏菌的检测灵敏度达到7.0×103cfu/ml,且特异性良好。[结论]试验选取的序列和引物具有较高的特异性,可用于肠炎沙门氏菌的特异性检测。[Objective] The research aimed to establish SDA rapid detection method of Salmonella enteritidis.[Method] Strand displacement amplification（SDA） primers were designed according to invA gene fragment of S.enteritidis to establish SDA rapid detection method of S.enteritidis.And the sensitivity and specificity of the designed primers were studied.[Result] SDA rapid detection method of S.enteritidis was successfully established and the target fragment was successfully amplified by using SDA method.The detection sensitivity of S.enteritidis by using the established SDA detection method reached 7.0 × 103 cfu/ml,and the reaction was of good specificity.[Conclusion] The selected sequences and primers had higher specificity,which could be used in the specificity detection of S.enteritidis.

关 键 词：肠炎沙门氏菌 链置换扩增 琼脂糖凝胶电泳 

分 类 号：S852.6[农业科学—基础兽医学]