黄连素对重症急性胰腺炎大鼠肠屏障功能的保护作用  

Protective Effect of Berberine on Intestinal Barrier Injury in Severe Acute Pancreatitis in Rats

作　　者：梁鸿寅[1] 李诗思[2] 闫洪涛[1] 黄竹[1] 刘卫辉[1] 王涛[1] 刘立业[1] 罗浩[1] 林宁[1] 戴睿武[1] 陈涛[1] 汤礼军[1] 

机构地区：[1]成都军区总医院全军普通外科中心,成都610083 [2]第三军医大学新桥医院心血管外科重症监护室,重庆400037

出　　处：《解放军医药杂志》2014年第7期1-3,15,共4页Medical & Pharmaceutical Journal of Chinese People’s Liberation Army

基　　金：国家自然科学基金(81000185);四川省青年科技创新团队基金项目(2011JTD0010)

摘　　要：目的观察黄连素是否对重症急性胰腺炎(SAP)大鼠模型肠屏障功能具有保护作用。方法将SD大鼠随机分为假手术组(SHAM组),手术未给药组(SAP组)和手术给药组(SAP+RC组),每组12只。SAP组术前5 d每天生理盐水灌胃1次,SAP+RC组术前5 d每天黄连素灌胃1次。手术造模后24 h取材,对大鼠胰腺及小肠进行组织病理学评分,测定大鼠血清中内毒素、二胺氧化酶及胰腺淀粉酶水平。结果 SAP组及SAP+RC组均造模成功。SAP组、SAP+RC组与SHAM组比较,胰腺、小肠组织病理学评分、血清胰淀粉酶、二胺氧化酶、内毒素均显著升高(P<0.05);与SAP组比较,SAP+RC组胰腺组织病理学评分、二胺氧化酶、内毒素均显著降低(P<0.05)。结论黄连素对SAP大鼠模型肠屏障功能具有明确的保护作用。Objective To observe the effect of berberine on intestinal barrier function in severe acute pancreati-tis （SAP） in rats. Methods A total of 36 Sprague-Dawley rats were randomly divided into sham operation group （group A, n = 12）, operation without medication group （group B, n = 12） and operation with medication group （group C, n =12）. Group B was lavaged with physiological saline （1 time/ d） 5 d before the operation. Group C was lavaged with ber-berine （1 time/ d） 5 d before the operation. The samples of successful models were taken out 24 h after model establish-ment, and levels of endotoxin, diamine oxidase and pancreatic amylase in the serum samples were measured. Results The pancreatitis models were established successfully in group B and C. Compared with those in group A, pancreatic and enteric pathological scores, levels of serum amylopsin, diamine oxidase and endotoxin in group B and C were significantly increased （P 〈 0. 05）; pancreatic pathological score, levels of serum diamine oxidase and endotoxin in group C were sig-nificantly decreased, compared with those in group B （P 〈 0. 05）. Conclusion Berberine can protect intestinal barrier function in severe acute pancreatitis （SAP） in rats.

关 键 词：胰腺炎 急性坏死性 肠黏膜 小檗碱 大鼠 

分 类 号：R576.1[医药卫生—消化系统]