口服重组减毒沙门菌TPIN抗小鼠黑色素瘤研究  

Effect of Oral Recombinant Attenuated Salmonella Typhimurium TPIN on Melanoma in Mice

作　　者：哈小琴[1] 张尚弟[1] 杨志华[1] 孙琳[1] 郭馨云[1] 彭俊华[1] 董菊子[1] 张媛媛[1] 

机构地区：[1]兰州军区兰州总医院检验科,兰州730050

出　　处：《解放军医药杂志》2014年第7期43-47,共5页Medical & Pharmaceutical Journal of Chinese People’s Liberation Army

基　　金：甘肃省科技重大专项(1302FKDA039);兰州市科技项目(2011-2-60);全国博士后基金(20060390192)

摘　　要：目的观察重组沙门菌TPIN对小鼠黑色素瘤治疗的作用。方法应用小鼠黑色素瘤B16细胞给16只C57纯系小鼠建立荷黑色素瘤动物模型;将模型组随机分为对照组(PBS组)及治疗组(TPIN),每组各8只。治疗组应用重组减毒沙门菌TPIN菌灌胃,对照组PBS缓冲溶液灌胃,每周1次共4次,在此期间记录瘤体体积。最后1次灌胃1周后,荷瘤小鼠心脏采血后处死,检测CD3+/CD4+、CD3+/CD8+的T淋巴细胞表型的变化,瘤组织γ-干扰素(IFN-γ)含量及各组织脏器CMV的表达水平。结果成功构建了荷瘤小鼠模型,与治疗组比较,对照组肿瘤体积差异有显著性(P<0.01);治疗组血CD3、CD3+/CD8+、肿瘤组织中IFN-γ表达量显著高于对照组(P<0.05,P<0.01),治疗组小鼠的肠、肾、肝、脾、胃、肿瘤组织中均可扩增出与CMV瘤体大小相符的片段而对照组未扩增出相应片段。结论口服DNA疫苗TPIN免疫荷黑色素瘤小鼠可激起小鼠体内有效的免疫反应并能有效抑制瘤体的生长。Objective To observe the effect of oral recombinant attenuated salmonella typhimurium TPIN on melanoma in mice. Methods Mouse models of melanoma were established in 16 only inbred mice C57 using cultured mouse melanoma B16 injection; the models were randomly divided into control group （ PBS group, n = 8） and TPIN treatment （TPIN group, n = 8）. TPIN group received gavage with recombinant Salmonella TPIN once a week for 4 times, while the PBS group was given phosphate buffered saline （PBS） with the same method, and tumor volumes were recorded during treatment. After treatment, heart blood of tumor-bearing mice was collected and the mice were sacrificed 1 week after the last gavage. The changes of CD3 ＋ / CD4 ＋ , CD3 ＋ / CD8 ＋ and T-lymphocyte phenotype were measured, and interferon-γ （IFN-γ） contents of tumor-bearing tissues and CMV expression levels of organs were detected. Results Mouse models of melanoma were successfully established. The differences in tumor volumes in TPIN group were statisti-cally significant, compared with those in PBS group （P 〈 0. 01）; levels of CD3 and CD3 ＋ / CD8 ＋ in blood, and IFN-γ expression in tumor tissue in TPIN group were significantly increased, compared with those in PBS group （P 〈 0. 05）. In TPIN group, CMV fragment could be amplified from intestine, kidney, liver, spleen, stomach and tumor tissues, while the fragment was not in the control group. Conclusion Oral TPIN DNA Vaccine can stimulate the effective im-mune action in melanoma mice, which can inhibit tumor growth of tumor-bearing mice as well.

关 键 词：白细胞介素 2 沙门菌菌苗 黑色素瘤 实验性 小鼠 

分 类 号：R739.5[医药卫生—肿瘤]