Survivin和Bcl-2蛋白在胃癌细胞中的表达及其与化疗药物耐药性关系的研究  

The relationship between Survivin and Bcl-2 expression and chemoresistance in gastric carcinoma

作　　者：周露婷[1] 王翠翠[1] 景洪标[1] 李培峰[1] 耿明[1] 

机构地区：[1]济南军区总医院病理科,山东济南250031

出　　处：《现代肿瘤医学》2014年第7期1627-1629,共3页Journal of Modern Oncology

基　　金：济南军区十一五计划课题(编号:J115Z004)

摘　　要：目的:检测Survivin和Bcl-2蛋白在胃癌组织中的表达,探讨Survivin和Bcl-2基因与胃癌化疗药物耐药性的关系。方法:取新鲜胃癌手术标本98例,免疫组化检测Survivin和Bcl-2蛋白在胃癌组织中的表达。同时进行原代胃癌细胞培养,MTT比色法检测原代培养细胞对化疗药物HCPT、CDDP、5-FU、ADM和MMC的敏感性。结果:胃癌组织中Survivin和Bcl-2蛋白的阳性率分别为74.5%和70.4%。Survivin阳性表达者对5-FU和ADM的耐药性显著高于阴性者,差异有统计学意义(P<0.05)。5-FU、ADM和MMC三种药物对Bcl-2阳性表达者的抑制率显著低于Bcl-2阴性表达者(P<0.05),其阳性表达与5-FU、ADM及MMC的体外耐药相关。结论:Survivin和Bcl-2基因与胃癌对化疗药物敏感性相关,Survivin与胃癌细胞原发性化疗耐受有关,检测其表达对选择化疗药物有实用价值。Bcl-2可能参与介导胃癌细胞的耐药,其高表达可能是胃癌多药耐药产生的原因之一。Objective：To detect the expression of Survivin and Bcl -2 in gastric carcinoma, and investigate the re- lationship between Survivin and Bcl - 2 expression and the drug - resistance to chemotherapeutic - drugs. Methods： All 98 samples of gastric cancer were collected, Survivin and Bcl - 2 were detected by immunohistochemistry. Primary single - cell suspensions were prepared from fresh specimens of primary gastric cancer and exposed to hydroxycampto- thecin （ HCPT ）, cisplatin （ CDDP）, 5 - fluorouracil （5 - FU）, adriamycin （ADM） and mitomycin （MMC） for 48 h. Cell metabolic activity and rate of inhibition were evaluated using tetrazolium（MTI＇） assay. Results： Positive rates of Sur- vivin and Bcl -2 in gastric cancer were 74.5 % and 70.4 %, respectively. Survivin positive cells showed more tolerant to 5 - FU and ADM than the negative cells （ P 〈 0.05 ） ; Inhibited rate of the Bcl - 2 positive cells was remarkably higher than the Bel - 2 negative cells in 5 - FU, ADM and MMC. Conclusion： Expression of Survivin and Bcl - 2 was correlated with primary drug -resistance of gastric cancer, and Survivin may be related with primary tolerance of gas- tric cancer to chemotherapeutic - drugs, which could be used as predictors in gastric carcinoma drugs administration. Bcl - 2 was likely intervene drug - resistance, and its high expression could be a cause of muhidrug resistance in gas- tric cancer.

关 键 词：胃肿瘤 免疫组织化学 SURVIVIN BCL-2 化疗药物 耐药性 

分 类 号：R735.2[医药卫生—肿瘤]