巨噬细胞表型在重症胰腺炎肾损伤中的表达  

The expression of macrophage phenotype in kidney injury caused by severe acute pancreatitis

作　　者：党胜春[1] 冯舒[1] 王平江[1] 沈耀[1] 张建新[1] 

机构地区：[1]江苏大学附属医院普外科, 江苏省镇江212001

出　　处：《中华急诊医学杂志》2014年第7期765-769,共5页Chinese Journal of Emergency Medicine

基　　金：国家自然科学基金（81070287）;江苏省自然科学基金（BK2011484、BK2012704）

摘　　要：目的 探讨巨噬细胞（Mφ）表型在重症急性胰腺炎（severe acute pancreatitis,SAP）急性肾损伤中的机制.方法 雄性Wistar大鼠64只,随机（随机数字法）分为对照组（SO组）和SAP组,每组32只,采用逆行胰胆管注射5％牛磺胆酸钠制备SAP大鼠模型,于造模后2、6、12及24h分别取血和肾组织.自动生化分析仪测定血液中尿素氮（BUN）、肌酐（Cr）的改变.逆转录-聚合酶链反应（RT-PCR）方法检测肾组织IL-12、TNF-α、IL-10及TGF-β的mRNA的表达水平.蛋白质印迹法检测CD68、iNOS、Arg-1的表达.结果 SAP组各时点BUN、Cr的浓度均高于对照组（P ＜0.01,P＜0.05）;SAP组各时点肾组织IL-12、TNF-α、IL-10、TGF-β的mRNA的表达水平较对照组显著增高,差异具有统计学意义（P＜0.01,P＜0.05）; SAP组各时点CD68、iNOS、Arg-1表达量均高于对照组.结论 SAP时炎症反应及炎症失衡是急性肾损伤的可能病理因素.Objective To investigate the macrophages （Mφ） phenotype mechanism in acute kidney injury caused by severe acute pancreatitis （SAP）.Methods Sixty-four male Wistar rats were randomly divided into control group （SO） and SAP group （n =32 in each group）.SAP rat model was made by retrograde cholangiopancreatic injection of 5％ sodium taurocholate.At 2,6,12 and 24 h after modeling,the samples of blood and kidney tissue were collected.The levels of blood urea nitrogen （BUN） and creatinine （Cr） were detected by using automatic biochemical analyzer.The expressions of IL-12,TNF-α,IL-10 and TGF-β mRNA of kidney tissue were detected by fluorescence quantitative polymerase chain reaction （QRT-PCR）.The levels of CD68,iNOS and Arg-1 were measured by Western blot.Results In the SAP group at each interval,BUN and Cr concentrations were significantly higher than those of the control group （P ＜ 0.01,P ＜ 0.05） ; Compared with the control group,the expressions of IL-12,TNF-α,IL-10 and TGF-β mRNA in renal tissue of SAP group were significantly higher （P ＜ 0.01,P ＜ 0.05）.In the SAP group,the levels of CD68,iNOS and Arg-1 were higher than those in the control group.Conclusions Inflammation and inflammatory imbalances may be pathological factors of acute kidney injury following SAP.

关 键 词：重症急性胰腺炎 肾损伤 巨噬细胞 CD68 

分 类 号：R576[医药卫生—消化系统]