莱菔硫烷对阿尔茨海默病小鼠脑组织Aβ沉积和氧化应激的影响  

Effects of sulforaphane on brain Aβ deposition and oxidative stress in Alzheimer＇s disease mice model

作　　者：左玥[1] 陈楠[1] 李熹[1] 赵越[1] 刘莉[1] 安丽[1] 

机构地区：[1]中国医科大学公共卫生学院营养与食品卫生学教研室,沈阳110001

出　　处：《中华老年医学杂志》2014年第7期799-801,共3页Chinese Journal of Geriatrics

摘　　要：目的 探讨莱菔硫烷（SFN）对阿尔茨海默病（AD）的干预作用. 方法 将8周龄C57BL/6J小鼠按体质量随机分为3组（n=10）.干预组、模型组小鼠饮用含铝水（0.4 g/100 ml）及隔日1次皮下注射200 mg/kg D-半乳糖,并每日1次分别灌胃25 mg/kg SFN和等量蒸馏水,同时设立溶媒对照组.90 d后检测血铝水平、脑组织Aβ沉积情况及大脑皮质氧化应激相关指标. 结果 模型组和干预组小鼠血铝水平高于对照组,干预组小鼠血铝水平低于模型组,差异均有统计学意义（P＜0.01）.与对照组比较,模型组小鼠脑组织Aβ沉积斑块增多,谷胱苷肽过氧化物酶活性降低,羰基含量增加（均P＜0.05）;而干预组小鼠脑组织Aβ沉积斑块、谷胱苷肽过氧化物酶活性及羰基含量与对照组比较,差异无统计学意义（P＞0.05）.与模型组比较,干预组小鼠脑组织Aβ沉积斑块减少（P＜0.05）.各组小鼠脑组织SOD活性及总巯基含量差异无统计学意义（P＞0.05）. 结论 SFN可降低AD模型小鼠的血铝水平,减少脑组织Aβ斑块沉积,并对氧化应激具有一定的调节作用.Objective To investigate the interventional effect of sulforaphane （SFN） on brain Aβ deposition and oxidative stress in Alzheimer＇s disease model mice.Methods Eight-week old C57BL/6J mice were randomly divided into three groups （n =10,each）.Mice in two groups （intervention and model）were administered by drinking water containing 0.4g aluminum /100ml water and subcutaneous injection with 200mg/kg D-galactose every other day,added with 25mg/kg SFN to the intervention group or with equivalent double-distilled water to model group by a single oral gavage daily.And equivalent vehicle were given to the control mice.After 90 days,the aluminum levels in blood,brain Aβ depositions,and indices of oxidative stress in cerebral cortex were investigated.Results Blood aluminum level was higher in the AD model and SFN intervention groups than in controls（P＜0.01）,and was lower in SFN intervention group than in AD model（P＜0.01）.As compared to controls,the AD model showed that brain Aβ deposition was significantly increased,GPX activity was decreased and carbonyl level was remarkable increased（P＜0.05）.There were no significant differences between intervention and control group in brain Aβ deposition,GPX activity and carbonyl contents （P＞0.05）.Brain Aβ deposition was lower in intervention than in model group（P＜0.05）.There was no significantly differences in brain SOD activity and total hydrosulfuryl content among three groups（P＞0.05）.Conclusions Sulforaphane can reduce the aluminum level in blood,attenuate Aβ deposition,and regulate the oxidative stress in cerebral cortex in AD model mice.

关 键 词：阿尔茨海默病 莱菔硫烷 淀粉样Β-蛋白 氧化性应激 

分 类 号：R749.16[医药卫生—神经病学与精神病学]