小白菊内酯诱导肝癌细胞SMMC 7721自噬性死亡的实验研究  

Feverfew Lactone Induces Autophagic Death of Hepatocellular Carcinoma SMMC 7721 Cells

作　　者：刘战培[1] 李艳艳[2] 高波[2] 黎军[2] 高俊萍[2] 林苹[2] 

机构地区：[1]中航工业三六三医院普外科,成都610041 [2]四川大学华西医院实验肿瘤研究室,成都610041

出　　处：《四川大学学报（医学版）》2014年第4期587-590,共4页Journal of Sichuan University(Medical Sciences)

摘　　要：目的探讨小白菊内酯对肝癌细胞SMMC 7721自噬反应的影响及其机制。方法通过MTT测定小白菊内酯对SMMC 7721细胞活力的影响;通过吖啶橙染色观察不同浓度小白菊内酯对SMMC 7721细胞自噬的影响,Western blot检测细胞自噬标志物LC3、p62的表达以及自噬流检测分析小白菊内酯对细胞自噬的影响;用抗氧化剂NAC去除细胞中ROS,通过Western blot检测小白菊内酯对细胞自噬的影响,确定ROS在其中的作用。结果小白菊内酯可抑制SMMC 7721细胞的增殖,并具有浓度依赖性;通过自噬的标志物及自噬流的检测显示小白菊内酯可诱导肝癌细胞系SMMC 7721细胞发生自噬,用NAC去除ROS后,小白菊内酯引起的自噬明显下降,说明其诱导的SMMC 7721细胞发生自噬依赖于ROS。结论小白菊内酯可能是通过刺激细胞产生ROS诱导SMMC 7721肝癌细胞发生自噬性死亡。Objective To explore the effect and mechanism of feverfew lactone on inducing autophagic death of hepatocellular carcinoma.Methods The proliferation of hepatocellular carcinoma SMMC 7721cells treated with feverfew lactone was measured by MTT assay.The autophagy of SMMC 7721induced with feverfew lactone was assessed by acridine orange staining,autophagic marker LC3and p62detecting and autophagic flows analyzing.In addition,a role of ROS in this process was stated by treatment with antioxidant agent N-acetyl-L-cysteine（NAC）.Results The proliferation of SMMC 7721cells were inhibited by feverfew lactone in a concentration dependence manner.The expression of LC3and autophagic flows of SMMC 7721cells were increased by feverfew lactone,while p62was decreased,which implied that feverfew lactone could induce the autophagy of SMMC 7721cells.Further more,the autophagy effect induced by feverfew lactone was declined obviously when traeted with NAC suggested that ROS played an important role in this effect.Conclusion Feverfew lactone induces autophagic death of SMMC7721cells by stimulating cells to produce ROS.The study will be helpful for the treatment of hepatocellular carcinoma and to provide theoretical basis for the clinical application of feverfew lactone.

关 键 词：小白菊内酯 肝细胞癌 自噬 活性氧 

分 类 号：R735.7[医药卫生—肿瘤]