大鼠颌骨来源骨髓间充质干细胞中Cbfα1/p56亚型的表达  

Expression of Cbfα1/p56 subtype in bone marrow mesenchymal stem cells from rat mandible

作　　者：周建华[1] 许艳彬[2] 邱建忠[1] 陈正岗[1] 江宏兵[2] 王莉莉[3] 

机构地区：[1]青岛大学附属青岛市市立医院口腔医学中心,山东省青岛市266011 [2]南京医科大学附属江苏省口腔医院,江苏省南京市210029 [3]青岛大学附属青岛市市立医院中心实验室,山东省青岛市266011

出　　处：《中国组织工程研究》2014年第23期3622-3626,共5页Chinese Journal of Tissue Engineering Research

基　　金：青岛市科技局项目支撑计划(KJZD-12-33-nsh)~~

摘　　要：背景:与来源于中胚层间充质的躯干四肢骨不同,颌骨来源于颅神经嵴外胚间充质,具有独特的膜内成骨机制。核心结合因子Cbfα1是骨分化的关键性转录因子,但其p56亚型在颌骨组织中的作用尚不明确。目的:研究Cbfα1/p56亚型在大鼠颌骨来源骨髓间充质干细胞中的表达及意义。方法:原代培养大鼠颌骨和髂骨来源的骨髓间充质干细胞,采用ELISA法检测骨髓间充质干细胞中的碱性磷酸酶活性,荧光定量PCR法检测骨髓间充质干细胞中Cbfα1/p56和p57亚型的mRNA相对表达量。结果与结论:成功培养出大鼠颌骨和髂骨来源的骨髓间充质干细胞,颌骨来源骨髓间充质干细胞的增殖能力及碱性磷酸酶活性明显高于髂骨;在培养第6天,颌骨来源骨髓间充质干细胞的Cbfα1/p56亚型mRNA相对表达量高于髂骨来源骨髓间充质干细胞(P<0.05),而Cbfα1/p57亚型在各培养时间点的mRNA相对表达量两组间差异均无显著性意义(P>0.05)。结果提示Cbfα1/p56亚型对颌骨来源骨髓间充质干细胞的早期成骨分化具有重要意义。BACKGROUND： Unlike the ilium derived from the paraxial mesoderm, the mandible from cranial neural crest has a unique mechanism. Core binding factor al （Cbfal） is a key transcription factor for skeletogenic process. However, the role of Cbfa1/p56 subtype in mandible tissue is yet not clear. OBJECTIVE： To research the expression of Cbfa1/p56 subtype in bone marrow mesenchymal cells from rat mandible in vitro. METHODS： Bone marrow mesenchymal stem cells from rat mandible and ilium were in vitro isolated and purified by primary culture. The characteristics of bone marrow mesenchymal cells were compared through the methods of enzyme linked immunosorbent assay and real-time PCR, including growth curve, alkaline phosphatase activity and relative mRNA expression of Cbfal subtypes. RESULTS AND CONCLUSION： Bone marrow mesenchymal cells from rat mandible and ilium were successfully obtained. Bone marrow mesenchymal ceils from the mandible proliferated more rapidly, alkaline phosphatase activity of which was higher than lilac cells. The relative mRNA expression of Cbfa1/p56 subtype in bone marrow mesenchymal cells from the mandible was more than that in lilac cells at 6 days of culture （P 〈 0.05）, while the expression of Cbfg1/p57 in each time showed no statistical significance （P 〉 0.05）. The results showed that Cbfa1/p56 is very significant in the eady osteogenic differentiation of bone marrow mesenchymal cells from the mandible.

关 键 词：干细胞 骨髓干细胞 核心结合因子Α1 亚型 p56 P57 骨髓间充质干细胞 成骨分化 颌骨 髂骨 

分 类 号：R394.2[医药卫生—医学遗传学]