Pharmacokinetic and tissue distribution studies of paeoniflorin and albiflorin in rats after oral administration of total glycosides of paeony by HPLC-MS/MS  

HPLC-MS/MS法研究白芍总苷在大鼠体内的药代动力学和组织分布(英文)

作　　者：赵敏敏[1] 高萌[1] 田玉路 杜英峰[1] 王春英[1] 许慧君[1] 张兰桐[1] 王巧[1] 

机构地区：[1]河北医科大学药学院药物分析教研室,河北石家庄050017

出　　处：《Journal of Chinese Pharmaceutical Sciences》2014年第6期403-411,共9页中国药学（英文版）

基　　金：Natural Science Foundation of Hebei Province of China(Grant No.C2011206158,08B031);Hundreds of Innovative Talents Project of Hebei Education Department of China

摘　　要：A simple, sensitive and specific high performance liquid chromatography-tandem mass spectrometry （HPLC-MS/MS）method combined with solid phase extraction has been developed and validated for the simultaneous quantification of paeoniflorinand albiflorin in rats plasma and tissue homogenate after administration of total glycosides of paeony （TGP）. Chromatographicseparation was achieved on a C15 column （150 mmx4.6 mm, 5 pro） with mobile phase consisted of acetonitrile-0.05% formicacid aqueous （20： 80, v/v） at a flow rate of 0.5 mL/min. The analytes were detected with triple-quadrupole mass spectrometer usingturbo ion spray with negative ionization in the multiple reaction-monitoring （MRM） modes. The results of method validationconformed to the requirements for the determination of biological samples. This method was successfully applied to thepharmacokinetics and tissue distribution study of TGP in rats. The results showed that paeonifiorin and albiflorin wereabsorbed and reached peak value quickly, and had the similar pharmacokinetic characteristics. Both of them underwent a rapid andwide distribution in the tissues throughout the whole body, among which stomach was the main distribution tissue. Moreover, theyhad the ability to cross the blood-brain barrier.建立HPLC-MS/MS法测定大鼠血浆和组织样品中芍药苷和芍药内酯苷,研究白芍总苷在大鼠体内的药代动力学和组织分布特征。采用固相萃取法进行样品处理,色谱柱为C18柱,流动相为乙腈–0.05%甲酸溶液(20:80,v/v),ESI源,负离子多反应监测模式。方法学验证结果均符合要求。白芍总苷灌胃给与大鼠后,主要成分芍药苷和芍药内酯苷迅速吸收并达到峰值,二者具有相似的药动学特征,在组织中分布广泛,并可透过血脑屏障。

关 键 词：Total glycosides of paeony Solid-phase extraction ALBIFLORIN PAEONIFLORIN HPLC-MS/MS 

分 类 号：R969[医药卫生—药理学]