滑桃树种子内生菌Streptomyces sp.WXC抗菌化合物的分离和结构鉴定  

Antifungal Compounds from Trewia nudiflora Seed Endophytic Streptomyces sp. WXC

作　　者：吴欣[1] 魏博[1] 

机构地区：[1]国家海洋局第三海洋研究所,厦门361005

出　　处：《天然产物研究与开发》2014年第7期990-993,1029,共5页Natural Product Research and Development

摘　　要：本文对分离自滑桃树种仁的内生菌WXC进行初步的分类研究,其形态特征和系统发育分析表明该菌属于链霉菌属(Streptomyces sp.)。其发酵产物具有抗菌活性,以抗菌活性为指导,采用硅胶层析、凝胶层析和反相中压柱层析对Streptomyces sp.WXC发酵代谢产物进行分离纯化,得到2个吡喃萘醌类化合物,通过核磁共振、质谱等波谱技术确定它们分别为frenolicin B(1)和deoxyfrenolicin(2)。Based on the morphological characteristics and phylogenie analysis, endophytie strain WXC isolated from the seed of Trewia nucliflora was classified as Streptomyces sp. The fermentation products of strain WXC showed antifungal activity. Bioactivity-guided fractionations resulted in the isolation of two pyran naphthoquinone compounds, using column chromatography. The structures of compounds 1 and 2 were elucidated respectively as frenolicin B and deoxyfrenoliein according to spectroscopic analyses including NMR and MS.

关 键 词：内生菌 STREPTOMYCES SP WXC 分离纯化 结构 

分 类 号：Q939.13[生物学—微生物学] R931.17[医药卫生—生药学]